H22肝癌与Lewis肺癌荷瘤小鼠证候特征比较研究

目的比较H22肝癌与Lewis肺癌C57BL/6N荷瘤小鼠证候及其差异。方法分别在雄性C57BL/6N小鼠右腋下接种H22肝癌腹水和Lewis肺癌瘤组织单细胞悬液,并设立正常对照组,采用小鼠四诊工作站平台与方法收集小鼠四诊信息,并进行计量辨证。结果接种第11~21天,Lewis肺癌小鼠肿瘤增长与小鼠死亡速度较H22肝癌小鼠速度快,第21天H22肝癌与Lewis肺癌小鼠存活比例分别为43.75%(7/16)和25.0%(5/20)。H22肝癌与Lewis肺癌荷瘤小鼠均自发形成了常见虚证,其中Lewis肺癌小鼠证候出现早,程度重,发展快。结论H22肝癌与Lewis肺癌C57BL/6N荷瘤小鼠与人类类似,存在着证候的自然发生与演变,可模拟临床肿瘤证候的发生与演变,用于辨证论治的实验研究;而本文所采用的计量化辨证有助于荷瘤小鼠个体化辨证论治,以及疾病和辨证论治客观化、计量化的疗效评价。

Comparative research on syndrome manifestation features in mice with H22 liver cancer and Lewis lung carcinoma

Objective To compare the difference of syndrome manifestation in C57BL/6N mice with H22 liver cancer and Lewis lung carcinoma.Methods Mice models with H22 liver cancer and Lewis lung carcinoma were established by inoculating H22 liver cancer ascites or cell suspension of Lewis lung carcinoma in armpit of C57BL/6N mice.C57BL/6N mice inoculated with normal sodium were taken as normal control. The four diagnostic methods workstation was used to collect the information which was adopted to practice quantitative syndrome differentiation.Results From the 11thd to the 21thd, the tumor growth and death speed of Lewis lung carcinoma mice were faster than those of H22 liver cancer mice. The survival rate was 43.75%(7/16)and 25.0 %(5/20)in H22 liver cancer mice and Lewis lung carcinoma mice on the 21th day.The asthenia syndrome developed spontaneously in both H22 liver cancer mice and Lewis lung carcinoma mice. And the asthenia syndrome in Lewis lung carcinoma mice occurred early and seriously, and developed quickly.Conclusion Just like the human being,H22 liver cancer mice and Lewis lung carcinoma mice present spontaneity and development of syndrome manifestation, which can be applied to imitate the development of syndrome manifestation in clinical tumor patients and can be used to study about the treatment based on syndrome differentiation. In addition, the quantitative syndrome differentiation adopted by us is helpful to treatment based on syndrome differentiation individually in tumor mice and to evaluate the curative effect objectively and quantitatively.