益气活血方药理血清对大鼠贮脂细胞增殖作用研究

研究益气活血方药理血清对大鼠贮脂细胞增殖作用机制。通过制备 3周、5周、7周的肝纤维化模型大鼠血清、正常大鼠血清和益气活血复方药物血清 ,培养肝星形细胞HSC(Hepaticstellatecells)和肝Bel74 0 2瘤株 ,采用MTT法观察和分析HSC的增殖作用。结果表明 :①益气活血方剂血清能抑制 5周、7周模型血清培养的继代HSC的增殖 ,P <0 .0 5。②益气活血方剂血清可促进 3周模型血清培养的继代HSC和模型血清培养的原代HSC的增殖 ,P <0 .0 5。③益气活血方剂血清和3周模型血清共同作用促进培养的Bel74 0 2的增殖 ,P <0 .0 5。提示益气活血方剂能够对HSC和Bel74 0 2细胞的增殖产生影响。HSC的生活状态 ,即HSC发育时相是决定益气活血方剂具有抑制或促进增殖作用的关键事件

A Study on Pharmacological Rat Serum with YiQiHuoXue on HSC Proliferation

Object: To study the hepatic stellate cell(HSC) proliferation in DMEM which contained rat sera with the recipe of YiQiHuoXue. Methods: Rat sera were taken respectively from normal rats, rats with recipe of YiQiHuoXue and rats model under liver fibrosis condition for 3, 5 and 7 weeks. Proliferating effect of HSC and Bel7402 cells in vitro were analyzed through MTT method. Results: 1. The sera with YiQiHuoXue can inhibit the proliferation of subculture HSC, which was cultured in model sera for 5 and 7 weeks, P <0.05. 2.The sera with YiQiHuoXue can promote the proliferation of subclture HSC which was cultured in model sera for 3 weeks and the proliferation of primary HSC which was cultured in model sera for 3,5 and 7 weeks, P <0 05.3.The sera with YiQiHuoXue can promote the proliferation of Bel7402 cell, which was cultured in model sera for 3 weeks, P <0.05.Conclusion: The recipe of YiQiHuoXue could infect the proliferation of HSC and Bel7402. HSC proliferation depends on whether HSC was inhibited or promoted.