艾烟冷凝物对肺泡Ⅱ型上皮细胞A549活性及凋亡的影响

目的观察艾烟冷凝物(PM10采取人体可吸入的艾烟部分)对肺泡Ⅱ型上皮细胞(A549)活性及凋亡的影响。方法体外培养A549细胞,加入不同浓度的艾烟冷凝物,应用四甲基偶氮唑蓝(MTT)法、荧光显微镜来观察其对A549的细胞活性以及细胞凋亡的影响。结果 MTT法结果显示:与对照组相比,A549的细胞存活率随浓度和时间发生改变,具有明显的时间浓度依赖性;0.12g/L浓度的艾烟冷凝物作用于细胞12 h后可显著提高细胞存活率(P=0.005<0.01);荧光显微镜下观察发现艾烟冷凝物可以引起细胞发生凋亡,且具有浓度依赖性。结论 A549细胞随着艾烟冷凝物浓度的增加、刺激时间的增加而引起细胞活力逐渐下降,表明一定浓度的艾烟冷凝物和一定的刺激时间对细胞具有毒性作用;一定浓度的艾烟冷凝物在较短刺激时间内具有细胞增殖作用,故认为艾烟对细胞的增殖作用可能是艾烟发挥有效作用的重要因素之一;能够引起细胞的凋亡可能是毒性作用的重要因素之一。

Influences of condensate of moxa smoke on viability and apoptosis of type Ⅱ alveolar epithelial cells (A549)

Objective To observe the influences of condensate of moxa smoke(PM10,taken inhalable portion of moxa smoke) on viability and apoptosis of type Ⅱ alveolar epithelial cells(A549).Methods A546 cells were cultured in vitro and then condensate of moxa smoke was added in different concentration.The influences of condensate of moxa smoke on the viability and apoptosis of A546 cells were observed by applying MTT assay and fluorescence microscope.Results The results of MTT assay showed that compared with control group,the survival rate of A546 cells changed with the changes of concentration and time showing an obvious time-dependence and a concentration-dependence.The condensate in the dose of 0.12 mg/mL significantly improved the survival rate of A546 cells after acting on the cells for 12 hours(P=0.005<0.01).The observation of fluorescence microscope showed that the apoptosis of A546 cells was induced by the condensate showing a concentration-dependence.Conclusion The viability of A546 cells decreases gradually as the increase of concentration and stimulating time of the condensate,which indicates that a certain concentration and a certain stimulating time of the condensate have some toxic effect on A546 cells.A certain concentration of the condensate can improve proliferation of A546 cells during a shorter stimulating time,which may be one of important factors of moxa smoke’s effect.The apoptosis of A546 cells may be one of important factors of moxa smoke’s toxic effect.