肠道菌群结构变化与高尿酸血症发生的关系

目的探讨肠道菌群结构变化在高尿酸血症(HUA)发生中的作用及可能机制。方法以雄性迪法克鹌鹑为研究对象,按体质量随机分为正常组、模型组、抗菌药物+正常饲料组(抗菌N组)、抗菌药物+高嘌呤饲料组(抗菌M组),正常组和抗菌N组喂食正常饲料,模型组和抗菌M组喂食高嘌呤饲料;正常组和模型组饮用自来水,抗菌N组和抗菌M组饮用含氨苄西林(150 mg/L)、新霉素(75 mg/L)的水,实验4周。PCR-DGGE法观察高嘌呤饮食和抗菌药物对鹌鹑肠道菌群结构,全自动生化仪或酶标仪检测细菌内毒素(LPS)、血尿酸(UA)及其关键酶黄嘌呤氧化酶(XO)的含量或活性。结果造模28 d,模型组鹌鹑肠道菌群结构发生变化,血浆及盲肠内容物中LPS含量、血清及肝脏中XO活性、血UA水平明显高于正常组(P0.05或P0.01);而抗菌N、M组中这些指标与正常组比较无显著性差异(P0.05);抗菌N、M组与模型组比较,其血浆及盲肠内容物中LPS含量减少,肝脏XO活性和血UA水平降低,差异均有统计学意义(P0.01或P0.05)。结论高嘌呤饮食可引起鹌鹑肠道菌群结构变化和HUA。肠道菌群代谢产物LPS的含量与XO活性、血尿酸水平呈正相关关系,HUA的发生机制可能与LPS激活XO活性有关。

Correlation between structural shifts of gut microbiota and hyperuricemia in quails

Objective To explore the effect and potential mechanism of gut microbiota on the development of hyperuricemia(HUA).Methods Altogether 48 Difake male quails were randomly diveded into four groups (n =12 each)according to body weight:normal group(N group),model group(M group),antibiotics and normal diet group(Ab +N group),and antibiotics and high-purine diet group(Ab +Mgroup).Quails in N group and Ab +N group were fed with normal diet,and those of other two groups were fed with high-purine di-et;at the same time,quails in N group and Mgroups drank clean tap water,and those of Ab +N group and Ab +M group drank water containing ampicillin (150 mg/L)and neomycin (75 mg/L)for consecutive 28 days.The microbiota structure of quail gut was determined by PCR-DGGE,while the concentration of endo-toxin (LPS)in plasma and cecal content,serum uric acid (UA),xanthine oxidase (XO)activity in serum and liver were measured by automatic biochemical analyzer or multiscan spectrum.Results Compared with N group,the gut microbiota structure of Mgroup changed,as well as the concentration of LPS in plasma and ce-cal contents,the activity of XO in serum and liver,and serum UA all increased significantly (P <0.05 or P <0.01);however,the indicators mentioned above showed no significant differences in Ab +N group and Ab +Mgroup (P >0.05).Compared with Mgroup,the concentration of LPS in plasma and cecal contents, the activity of XO in liver,and serum UA in Ab +N group and Ab +Mgroup all decreased dramatically(P <0.05 or P <0.01).Conclusion High-purine diet induced structural shifts of gut microbiota and HUA in quails.The concentration of LPS,metabolite of gut microbial flora,is positively related to the activity of XO and serum concentration of UA.And the occurrence of hyperuricemia maybe is attributed to activation effect of LPS on XO activity.