脑疏宁对颅脑创伤后大鼠血脑屏障通透性及MMP-9表达的影响

目的观察中药脑疏宁对创伤性脑损伤(TBI)大鼠血脑屏障(BBB)通透性及脑组织基质金属蛋白酶-9(MMP-9)蛋白表达的影响,探讨中药保护血脑屏障的作用机理.方法按Feeney自由落体撞击法造成TBI模型,采用免疫组织化学技术观察大鼠脑组织MMP-9表达.结果模型组创伤后24h BBB破坏,脑组织含水量增加,病灶区MMP-9蛋白表达增高,脑疏宁组BBB通透性、脑组织含水量及MMP-9表达较模型组降低(P＜0.01).结论颅脑损伤后MMP-9表达增高,促使BBB破坏,导致血管源性脑水肿.中药脑疏宁可以保护BBB,降低脑组织含水量,其作用可能与抑制MMP-9在损伤脑组织中的表达有关.

Effects of Naoshuning on permeability of blood-brain barrier and MMP-9 expression in TBI rats

Objective To investigate the effect of Naoshuning on permeability of blood-brain barrier (BBB) and protein expression of matrix metalloproteinase-9 (MMP-9) in rats with traumatic brain injury (TBI), and to discuss the protective mechanism of Chinese medicine on BBB. Method The model of TBI was established by bumpiness of free falling body. Immunohistochemistry was used to observe the brain protein expression of MMP-9.Result BBB was damaged and the hydrous content of brain and the protein expression of MMP-9 were significantly increased at 24h after TBI in contusion tissue in the model group. The permeability of BBB, hydrous content of brain and the MMP-9 expression were significantly decreased in the Naoshuning group (P<0.01, P<0.01, P<0.01).Conclusion A higher MMP-9 expression can damage BBB and induce the cerebral edema after TBI. Naoshuning can protect BBB and decrease the hydrous content of brain. The mechanism may be related to its inhibiting effect on the expression of MMP-9 of TBI.