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比较虚寒证与虚热证模型大鼠肝全基因表达谱的差异
引用本文:韩冰冰,王世军.比较虚寒证与虚热证模型大鼠肝全基因表达谱的差异[J].北京中医药大学学报,2011,34(10).
作者姓名:韩冰冰  王世军
作者单位:山东中医药大学基础医学院 山东 250355;山东中医药大学针灸推拿学院
基金项目:国家重点基础研究发展计划(973计划)资助项目(No.2007CB512601)
摘    要:目的采用基因芯片技术研究虚寒证与虚热证大鼠肝全基因表达谱的差异。方法使用中药复方虚寒证、虚热证大鼠模型,基因芯片检测各组大鼠基因表达,筛选差异表达基因,进行基因功能分类注释,荧光定量PCR验证芯片结果。结果虚寒模型组与虚热模型组比较有114条基因差异表达,主要涉及氧化还原酶相关基因。脂代谢相关氧化还原酶基因显著下调,细胞色素P450家族基因显著下调,提示虚寒证模型大鼠脂代谢与生物转化能力的降低。结论虚寒证与虚热证虽然同为虚证,但有寒证和热证不同的表现,氧化还原酶活性相关基因的异常表达可能是虚寒证与虚热证出现不同症状的物质基础。

关 键 词:基因芯片  虚寒证  虚热证  大鼠

Difference of all gene expression profiles of rat liver between model of deficient cold syndrome and model of deficient heat syndrome
HAN Bing-bing,WANG Shi-jun.Difference of all gene expression profiles of rat liver between model of deficient cold syndrome and model of deficient heat syndrome[J].Journal of Beijing University of Traditional Chinese Medicine,2011,34(10).
Authors:HAN Bing-bing  WANG Shi-jun
Institution:HAN Bing-bing1,WANG Shi-jun2(1 College of Basic Medical Sciences,Shandong University of Traditional Chinese Medicine,Shandong 250355,2 College of Acupuncture-Moxibustion and Tuina,Shandong University of Traditional Chinese Medicine)
Abstract:Objective To study the difference of all gene expression profiles of rat liver between model of deficient cold syndrome and model of deficient heat syndrome by using gene chip technology.Methods The rat model of deficient cold syndrome or deficient heat syndrome was established through giving TCM compound formulas.The gene expressions in rats of all groups were detected by using gene chips for screening the genes with differential expressions.The genetic function was commented according their classification...
Keywords:gene chips  deficient cold syndrome  deficient heat syndrome  rats  
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