乌拉尔甘草水溶性成分的HPLC指纹图谱研究

目的 建立乌拉尔甘草水溶性成分的HPLC指纹图谱,为有效控制乌拉尔甘草药材及甘草浸膏的质量奠定基础.方法 采用RP-HPLC法,色谱柱为Waters Sunfire~(TM) C_(18)(4.6 mm×150 mm,5 μm),以甲醇-0.1%磷酸水为流动相进行梯度洗脱,流速1.0 mL/min,检测波长260 nm,柱温25℃,检测时间95 min,分析了乌拉尔甘草主产区的12批样品.结果 在选定的色谱条件下,通过相似度分析确定18个色谱峰构成了乌拉尔甘草水溶性成分指纹图谱的特征峰,对其中的4个色谱峰进行了指认.结论 建立的乌拉尔甘草水溶性成分指纹图谱具有简便、稳定、可靠的优势,为乌拉尔甘草药材有效物质研究及甘草浸膏的质量控制奠定了基础.

HPLC fingerprint chromatogram of water-soluble constituents of Wula'er Gancao(Glycyrrhiza uralensis Fisch)

Objective To set up an an analysis method of fingerprint chromatogram of the water-soluble constituents of Wula'er Ganeao(Glycyrrhiza uralensis Fisch)by HPLC,and lay a foundation for the quality control of medical material of Wula'er Gancao and Gancao Extractum.Methods The procedure of RP-HPLC was performed on a chromatographic column of Waters Sunfire~(TM)C_(18)(4.6 mm×150 mm,5μm),using methanol-phosphoric acid water solution(0.1%)as the gradient elution,and the mobile phase was at flow rate of 1.0 mL/min.The detection wavelength wag at 260 nm and the analysis time was95 minutes at the temperature of 25℃.HPLC fingerprint chromatograms of 12 batches of Wula'er Gancao samples from main production area were analyzed.Results Under the above chromatographic conditions,18 chromatographic peaks consisted of the characteristic peak of fingerprint ehromatogram of the watersoluble constituents of Wula'er Gancao through similarity analysis,among which 4 were identified.Conclusion The fingerprint ehromatogram of water-soluble constituents of Wula'er Gancao set up by RPHPLC is simple,stable and repeatable,which can be used to lay a foundation for the quality control of medical material of Wula'er Gancao and Ganeao Extractum.