白花蛇舌草对巨噬细胞Cxcl1基因表达的影响及机制的初步研究

目的通过研究白花蛇舌草对巨噬细胞细胞因子表达的影响及Toll样受体4(TLR-4)炎性信号转导通路的影响,探索白花蛇舌草的免疫调节作用,为新药研发提供实验和理论基础。方法用白花蛇舌草水提液作用小鼠腹腔巨噬细胞,RT-PCR法检测3种常见细胞因子白细胞介素6(IL-6)、单核细胞趋化因子1(MCP-1)、Cxcl1的表达。然后用Autodock软件分析白花蛇舌草主要成分与TLR-4的相互作用,得出白花蛇舌草的主要活性物质对于TLR介导的信号通路的作用机理,从而得出白花蛇舌草对巨噬细胞炎症因子释放以及吞噬功能的影响。结果白花蛇舌草能上调Cxcl1基因的表达,其主要成分豆甾醇-β-D-葡萄糖苷、反式4-甲基肉桂醇、车叶草苷酸可以与TLR-4受体结合。结论白花蛇舌草可以激活TLR-4信号通路,从而影响Cxcl1的表达及巨噬细胞功能,因此推测白花蛇舌草有调控免疫的作用。

Effect and mechanism of oldenlandia on Cxcl1 gene expression in macro-phages

Objective To explore the immunoregulatory effect of Oldenlandia ( Hedyotis diffusa Villd.) , by investigating on the expression of macrophage cytokines and on TLR-4 inflammatory signal transduction pathway , aiming to propose a new experimental and theoretical evidence for the use of oldenlandia . Methods Three common cytokines , such as IL-6 , MCP-1 and Cxcl1 in macrophage cells were detected by using RT-PCR after treatment with oldenlandia solution .Autodock software was used to analyze the correlation between the main components and TLR-4 so as to explore the mechanism of expression chan-ges of the cytokines.Results Oldenlandia solution could upregulate the expression of Cxcl 1, which might depend on the combination of TLR 4 and stigmasterol-beta-D-glucoside , trans-4-methoxycinnamyl alcohol , and asperulosidic acid .Conclusion Oldenlandia could activate TLR-4 signal pathway and thus affect the expression of Cxcl 1 and function of macrophage .It can be inferred that oldenlandia could regu-late immunity , which could serve as the theoretical rationale for the development of new drugs in the labo-ratory.