应用差异显示筛查冠心病血瘀证相关基因及分析

目的探讨冠心病血瘀证证候基因组学研究方法。方法临床选择冠心病血瘀证患者,采用外周血mRNA差异显示结合反向Northern法,筛查病证结合证候相关基因,并进行临床验证。结果差显出现95条差异条带,经反向Northern后得到28条真实差异片段,经克隆、测序,去除相同序列,通过生物信息学分析,其中有5条与人类98%以上同源的基因序列,经相关文献资料和临床验证结果分析,它们与冠心病血瘀证病理改变密切相关。结论差异显示结合反向Northern和临床验证的方法,适用于筛查病证结合证候基因的研究。

Screening and analyzing correlative genes in blood stasis syndrome of CHD with differential display

Objective To investigate the genomic research methods in syndrome manifestations of blood stasis syndrome of coronary heart disease CHD).Method To choose the patients with blood stasis syndrome of CHD and control patients,and screen out the genes correlated to the disease and syndrome manifestations by differential display of peripheral blood mRNA combined with the method of Reserve Northern and the genes were tested in clinic.Result There were 95 differential strips in the differential display and among them 28 were determined as the real differential fragments.After cloning,sequence examination,remove of same sequence and analysis of bioinformatics there were 5 gene sequences with more than 98% homologous gene sequence with that of mankind.They were correlated to the pathological changes of blood stasis syndrome of CHD after the analysis on related documents,materials and clinical test outcomes.Conclusion Combination of differential display,Reserve Northern and clinical test was fit for the research of screening the genes of disease and(syndrome) manifestations.