山茱萸总甙对正常小鼠T淋巴细胞免疫功能影响的实验研究

研究了从山茱萸中提取的山茱萸总甙对正常小鼠T淋巴细胞及其亚群和产生IL 2的影响 ,以期探讨山茱萸总甙的免疫抑制机理。结果表明 :山茱萸总甙不同剂量均可抑制T淋巴细胞增殖(P <0 0 1)、T淋巴细胞膜表面CD3的表达 (P <0 0 1)和抑制T淋巴细胞膜表面CD4、CD8的表达(P <0 0 5或P <0 0 1) ,中大剂量还可以提高CD4 /CD8的比值 ,说明山茱萸总甙抑制CD8的作用比抑制CD4的作用更明显。不同剂量的山茱萸总甙还可以抑制T淋巴细胞产生IL 2 (P <0 0 5或P <0 0 1)。抑制IL 2的产生可能是山茱萸总甙抑制机体免疫功能的主要机理之一。

The Effects of the Total Glycoside from Cornus Officinalis on Immunologic Function of T Lymphocytes in Healthy Mice

The effects of the total glycoside extracted from Cornus officinalis (TGECO) on T lymphocytes and T lymphocyte subgroup, and the IL 2 production of T lymphocytes were studied in healthy mice in order to explore the immunosuppressive mechanism of TGECO. The results showed that all the different TGECO doses could inhibit the multiplication of T lymphocytes ( P <0 01), CD3 surface expression in T lymphocytes ( P <0 01), and CD4 and CD8 surface expression in T lymphocytes ( P <0 05 or P <0 01); besides, middle and large TGECO doses could increase the ratio of CD4/CD8, which implies that the TGECO effect of inhibiting CD8 is stronger than that of inhibiting CD4. The results also showed that all the different TGECO doses could inhibit the IL 2 production of T lymphocytes ( P <0 05 or P <0 01), which may be one of the main causes of the immunosuppressive effects of TGECO.