应用光学相干层析成像技术对脾胃湿热证大鼠舌象的量化分析研究

目的应用光学相干层析成像设备对脾胃湿热证大鼠的舌苔及舌质进行在体的显微层析成像与舌象指标的量化研究,探讨脾胃湿热证模型舌象在显微检测方面的变化规律以及光学相干层析成像于舌诊应用中的价值。方法采用复合因素复制脾胃湿热证大鼠模型,应用自行装置的光学相干层析成像设备,以LabVIEW编写软件操作平台,进行大鼠舌上表面的在体显微成像检测,并对舌苔的相关参数进行量化处理分析。结果从大鼠舌表面显微成像中能够清楚地分辨出舌苔层、舌苔舌体连接层与舌体层结构,与组织学检测大鼠舌显微结构结果显示具有一定的相似性;脾胃湿热证大鼠的舌象各部位的舌苔厚度明显高于正常对照组(P<0.01),而舌体部津液则明显低于正常对照组(P<0.01)。结论脾胃湿热证模型的舌象在显微检测上具有特征性的改变,光学相干层析成像技术在大鼠舌象中能进行成像研究,并可能实现一定的量化研究。

Quantitative analysis on tongue manifestations in rats with spleen-stomach damp-heat syndrome by optical coherence tomography

Objective To study quantitatively the indexes of microscopic tomography of tongue manifestations and tongue coating and texture in the rats with spleen-stomach damp-heat syndrome in vivo by applying optical coherence tomography(OCT),and to investigate the change laws of the tongue manifestations of spleen-stomach damp-heat syndrome in microscopic detection and value of the application of optical coherence tomography in tongue diagnosis.Methods The rat model of spleen-stomach damp-heat syndrome was established by using combined factors,and rat tongue surface was detected in vivo by microscopic tomography and the relevant parameters of tongue coating were processed and analyzed quantitatively on Lab VIEW software platform by applying self-made OCT equipment.Results The tongue coating layer and connection layer between tongue coating and body were observed clearly by microscopic tomography,which was similar to the result of microstructure of rat tongue by histological detection.The tongue coating was thicker significantly in the rats with spleen-stomach damp-heat syndrome than that in the normal rats(P<0.01),while the fluid of tongue body was less significantly(P<0.01).Conclusion The tongue manifestations of the model of spleen-stomach damp-heat syndrome have characteristic changes in microscopic detection.OCT technology can be used in the study of rat tongue manifestations quantitatively.