肝纤维化肝气郁结证大鼠模型研究与证候时间窗方剂验证

目的为进一步研究肝纤维化相关机制及中医证候,建立中西医病证结合的肝纤维化大鼠模型。方法采用四氯化碳皮下注射法建立的肝纤维化肝气郁结证大鼠模型为研究对象,通过体重、旷场行为学测定、抓力测试、宏观表征采集等方法进行大鼠证候判定,病理进行肝纤维化疾病判定,并使用柴胡疏肝散、逍遥散、四逆散等方剂对证治疗,秋水仙碱对病治疗。结果多数肝纤维化大鼠造模4周后出现肝气郁结证,并且持续至造模12周;病理变化为造模4周多为肝纤维化S2~S3期,造模8周多为S3期,造模12周多为S4期;方剂反证肝气郁结证症状及病理结果有不同程度改善。结论该造模方法建立的肝纤维化肝气郁结证证候时间窗约为4~10周,方剂反证有一定效果,该病证结合模型建立基本成功。

Study of liver fibrosis rat model with pattern of liver-qi stagnation and validation of pattern time window by TCM formulas treatment

Objective To explore the pathogenesis and TCMpatterns related to liver fibrosis ,as well as to establish the rat model of liver fibrosis of combination of disease and pattern.Methods The rat liver fibrosis model with pattern of liver-qi stagnation was induced by subcutaneous injection of carbon tetrachloride.The patterns were distinguished by body weight,open field test,grip strength test,and form collection of symptoms and signs,and the pathological stage was confirmed by HE and Masson staining.Then Chaihu Shugan Powder,Xiaoyao Powder and Sini Powder were used to direct at TCMpat-terns,and colchicine was used to direct at the disease.Results Four weeks after modeling,pattern of liver-qi stagnation occured on the majority of liver fibrosis rats and lasted to the twelfth week.On the oth-er hand,the main pathological stage was S2 to S3 at the fourth week after modeling,S3 at the eighth week and S4 at the twelfth week.The treatment of TCMformulas validated the improvement of symptoms and pathological stages.Conclusion The time window of TCM pattern of liver fibrosis rat model with pattern of liver-qi stagnation was from 4 to 10 weeks,which was proved by TCMformulas.And the model of combination of pattern and disease was reliable.