黄芪注射液对KKA~y小鼠肾小管上皮细胞转分化的影响

目的探讨黄芪注射液治疗肾间质纤维化的作用机制,为临床中药治疗糖尿病肾间质纤维化提供实验依据。方法将16只2型糖尿病模型KKAy小鼠随机分为模型组和黄芪注射液治疗组,另取8只C57BL/6J小鼠作为正常组,治疗组小鼠14周龄开始腹腔注射黄芪注射液。于24周龄处死小鼠,取肾脏分别观察肾组织病理形态学变化,免疫组织化学法检测小鼠肾组织中α-平滑肌肌动蛋白、钙依赖性跨膜糖蛋白和转化生长因子β1的表达。结果可见模型组发生明显的纤维化改变,而经黄芪注射液治疗后,小鼠肾间质基质轻度增多,病变较轻微。治疗组KKAy小鼠肾组织中α-平滑肌肌动蛋白、转化生长因子β1的表达较模型组减少,钙依赖性跨膜糖蛋白的表达增多。结论黄芪注射液可在一定程度上减少α-平滑肌肌动蛋白的表达,抑制KKAy小鼠肾脏组织中转化生长因子β1表达,上调钙依赖性跨膜糖蛋白的表达水平,阻断并逆转肾小管上皮-间质转化的过程,延缓肾小管上皮细胞转分化的发生发展进程。

Effects of Astragalus mongholicus injection on epithelial-mesenchymal transition of renal tubular epithelial cells in diabetic KKAy mice

Objective To observe the effect of Astragalus mongholicus injection on the expression of epithelial-mesenchymal transition-related proteins in KKAy mice with renal interstitial fibrosis. Methods Sixteen KKAy mice were randomly divided into model group ( n =8 ) and Astragalus mongholicus injection treatment group (n=8),and 8 C57BL/6J mice were used as normal control.The mice in treatment group were intraperitoneally given Astragalus mongholicus injection from the 14 th week. The fasting glucose levels were observed at the 16th, 20th, 24th week.The mice were sacrificed at the 24th week.The renal pathological changes were observed under light microscope.The expression of alpha-smooth muscle actin,E-cad and transforming growth factor β1 in the renal tissues were examined by immunohistochemical staining.Results Fibrosis was found in the KKAy mice of model group,while the mice in treatment group showed a slight increase in renal interstitium.Treatment with Astragalus mongholicus injection decreased the protein expression levels of alpha-smooth muscle actin,transforming growth factor β1 in the kidney tissues as compared with those in model group.Conclusion Astragalus mongholicu injection reduces the expression of alpha-smooth muscle actin and restrains the protein expres-sion of transforming growth factorβ1 in the kidney tissue of KKAy mice.These changes may inhibit and reverse the process of epithelial-mesenchymal transition,thus delaying the occurrence and development of renal interstitial fibrosis.