滋阴息风方对自发性高血压大鼠肾脏JAK/STAT信号通路的影响?

目的观察滋阴息风方对自发性高血压大鼠血压及肾脏JAK/STAT信号通路的影响。方法随机将40只SHR大鼠分为模型组、中药低剂量组、中药高剂量组和复方罗布麻组,10只具有相同背景的SKY大鼠做正常组。治疗组给予对应药物灌胃,正常组和模型组给予等量生理盐水灌胃,连续给药42 d,分别于给药后第0、7、14、21、28、35、42天采用小动物无创血压仪检测各组大鼠收缩压血压变化情况。实验第42天,于灌胃2 h后取材,全自动生化分析仪检测血清肌酐(Cr)、尿素氮(BUN)、尿酸(UA)含量。酶联免疫吸附试验(ELISA)法检测血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量,蛋白免疫印迹(Western blot)法检测肾脏p-JAK1、p-STAT1蛋白表达。结果与正常组比较,模型组大鼠血压、Cr、BUN、UA、IL-6、TNF-α显著升高,经治疗后,各项指标均有显著改善(P0.05、P0.01),以中药高剂量和复方罗布麻效果较为明显。Western blot结果显示,与正常组比较,模型组大鼠肾脏p-JAK1、p-STAT1蛋白表达显著升高(P0.01),与模型组比较,各治疗组p-JAK1、p-STAT1蛋白表达均有不同程度下降(P0.05,P0.01)。结论滋阴息风方对自发性高血压大鼠降压作用明显,可明显改善高血压肾脏功能损伤,具体机制可能是通过抑制JAK/STAT信号通路的激活实现。

Influences of Ziyin Xifeng Fang on the JAK/STAT signal pathway in the kidney of spontaneously hypertensive rats

Objective To observe the effects of Ziyin Xifeng Fang ( ZYXFF) , a Chinese medicine com-pound formulated by the method of nourishing yin and extinguishing wind, on the blood pressure and renal JAK/STAT signal pathway in the rat model of spontaneously hypertension ( SH) . Methods 40 SH rats were randomly divided into four groups ( n=10 each):model group, low dose of ZYXFF( low-dose) group, high dose of ZYXFF ( high-dose) group and compound apocynum group. Another 10 SKY rats with the same background were included in the control group. The rats of three treatment groups were ad-ministered intragastrically ( i. g. ) proper drugs, and those of control group and model group were i. g. sa-line for consecutive 42 days. The systolic blood pressure ( BP) was measured by using small animal non-invasive blood pressure meter at Day 0, 7, 14, 21, 28, 35 and Day 42. Two hours after i. g. at the last day of medication, all the rats were sacrificed. The serum contents of creatinin ( Cr) , blood urea nitrogen ( BUN) , and uric acid ( UA) were determined. The serum contents of IL-6 and TNF-αwere detected by ELISA assay, and the protein expression of renal p-JAK1 and p-STAT1 were measured by Western blot. Results Compared with control group, BP, Cr, BUN, UA, IL-6 and TNF-α increased significantly in model group, and the all indexes of the rats of the three medication groups decreased remarkably ( P<0. 05 or P<0 . 01 ) , especially the effects of high dose of ZYXFF and compound apocynum were stronger than those of low dose ZYXFF. Compared with control group, in model group rats renal p-JAK1, p-STAT1 protein expression significantly increased ( P<0 . 01 ) . The protein expressions of p-JAK1 and p-STAT1 decreased in the three medication groups compared with the model group ( P <0 . 05 or P <0 . 01 ) . Conclusion The mechanism of ZYXFF lowering blood pressure and attenuating the renal function impairment should be by the way of inhibiting the activation of JAK/STAT signal pathway.