抗呆1号对脑缺血再灌注小鼠海马星形胶质细胞GFAP表达的影响

应用免疫组织化学方法探讨抗呆Ⅰ号对小鼠前脑缺血再灌注损伤后海马星形胶质细胞表达GFAP动态变化的影响,其与缺血性神经元的联系.结果显示:前脑缺血再灌注1 d只有少数星形胶质细胞表达GFAP,再灌注3 d表达GFAP的阳性细胞数增加,再灌注7～10 d表达GFAP的阳性细胞数达到高峰,同时,可见细胞反应性胶质化特征,前脑缺血再灌注15 d GFAP阳性反应细胞数仍维持在较高水平.用药组GFAP的表达细胞数较模型组明显减少.而正常对照组和假手术组只有少数星形胶质细胞表达GFAP.且星形胶质细胞表达GFAP的强弱与神经细胞的存亡有关.说明前脑缺血再灌注后海马反应性星形胶质细胞表达GFAP呈动态变化,抗呆Ⅰ号对缺血再灌注损伤星形胶质细胞和神经细胞有良好的保护作用.

Effects of Kangdai No.1 on the Expression of GFAP of Hippocampal Astrocytes in the Mice Undergone Forebrain Ischemia and Reperfusion

The effects of Kangdai No. 1 on the dynamic changes in the expression of GFAP of hippocampal astrocytes in the mice with injury caused by forebrain ischemia and reperfusion, and the relationship between the effects of Kandai No. 1 and ischemic neurons were investigated by the immunohistochemical method. The model group showed that there was only a small number of GFAP-expressing astrocytes in the mice in 1 day after ischemia and reperfusion, there was an increase in the number of GFAP-expressing astrocytes in 3 days after ischemia and reperfusion, the number of GFAP-expressing astrocytes reached a peak in 7 - 10 days after ischemia and reperfusion, and the number of GFAP-ex-pressing astrocytes still remained a relatively high level in 15 days after ischemia and reperfusion; while the Kangdai No. 1 group showed that there was a marked decrease in the number of GFAP-expressing astrocytes; the normal control group and sham operation group showed that there was only a few GFAP-expressing astrocytes; and the changes in the numbers of GFAP-expressing astrocytes were related with the survival state of neurons. The results indicated that there was a dynamic change in the number of GFAP-expressing astrocytes when forebrain ischemia and reperfusion happened, and Kangdai No. 1 had good protective effects against the injury of astrocytes and neurons caused by forebrain ischemia and reperfusion.