针刀松解法、电针对膝骨关节炎兔中枢单胺类神经递质的影响

目的 对比观察针刀松解法、电针对膝骨关节炎兔中枢神经5-羟色胺类和儿茶酚胺类神经递质的影响.方法 采用高效液相色谱仪分别测定端脑、下丘脑、脑干和脊髓中5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、去甲肾上腺素(NA)、高香草酸(HVA)和3,4-二羟基苯乙酸(DOPAC)含量.结果 模型组端脑、下丘脑、脑干和脊髓等的5-HT含量与正常组相比均无显著性差异,但有降低的趋势;模型组下丘脑和脑干中5-HIAA含量与正常组相比无显著性差异,但端脑和腰膨大5-HIAA含量较正常组显著降低,针刀治疗后端脑或脊髓5-HIAA含量与模型组相比显著升高;电针治疗后端脑5-HIAA含量与模型组相比显著升高,而脊髓中5-HIAA含量与模型组相比显著降低.模型组端脑NA含量较正常组显著降低;针刀或电针治疗后NA含量与模型组相比较呈显著性升高;模型组下丘脑和脑干NA含量较正常组显著性增加,针刀或电针治疗后显著降低;模型组脊髓NA含量与正常组相比较无显著差别.模型组端脑HVA含量较正常组显著降低,针刀或电针治疗后显著性升高,针刀组与电针组相比较无显著性差别;模型组下丘脑、脑干和脊髓HVA含量与正常组比较无显著差别.模型组端脑和脊髓DOPAC含量与正常组相比无显著变化;模型组下丘脑和脑干DOPAC含量较正常组明显升高,针刀或电针治疗后DOPAC含量较模型组均显著降低.结论 针刀松解法或电针疗法对膝骨关节炎兔脑、脊髓中5-羟色胺类和儿茶酚胺类神经递质的含量有一定的调节作用,提示2种疗法在减轻关节软骨损伤的同时,可通过调节中枢神经5-羟色胺和儿茶酚胺类神经递质的合成和代谢以缓解关节疼痛的发生和发展.

Effects of acupotome dissolution therapy and electro-acupuncture on central monoamine neurotransmitters in rabbits with knee osteoarthritis

Objective To compare and observe the effects of acupotome dissolution therapy and electro- acupuncture on central neurotransmitters of 5-hyproxytryptamines (5-HT) and catecholamine (CA) in rabbits with knee osteoarthritis. Method The content of 5-HT, 5-hydroxyindole acetic acid (5-HIAA), noradrenaline (NA), homovanillic acid (HVA) and 3, 4-dihydroxyphenylacetic acid (DOPAC) in the endbrain, hypothalamus, brain stem and spinal cord were determined by applying HPLC. Result There was no significant difference but a downward trend in the content of 5-HT in the endbrain, hypothalamus, brain stem and spinal cord between the model group and normal group. There was no significant difference in the content of 5-HIAA in the hypothalamus and brain stem between the model group and normal group. The content of 5-HIAA in the endbrain and spinal cord decreased significantly in the model group compared with that in the normal group, increased significantly in the acupotome group compared with that in the model group, increased significantly in the endbrain in the electro-acupuncture group compared with that in the model group, and decreased significantly in the spinal cord in the electro- acupuncture group compared with that in the model group. The content of NA in the endbrain decreased significantly compared with that in the normal group, increased significantly in the acupotome group or electro-acupuncture group compared with that in the model group, increased significantly in the hypothalamus and brain stem in the model group compared with that in the normal group, and decreased significantly in the acupotome group or electro-acupuncture group. There was no significant difference in NA content in the spinal cord between the model group and normal group. The content of HVA in the endbrain decreased significantly in the model group compared with that in the normal group, and increased significantly in knife group or electro-acupuncture group. There was no significant difference in HVA content in the endbrain between the acupotome group and electro- acupuncture group. There was no significant difference in HVA content in the hypothalamus, brain stem or spinal cord between the model group and normal group. The content of DOPAC in the endbrain or spinal cord had no significant changes in the model group compared with that in the normal group, increased significantly in the hypothalamus or brain stem in the model group compared with that in the normal group, and decreased significantly in the acupotome group or electro-acupuncture group compared with that in the model group. Conclusion The acupotome dissolution therapy and electro-acupuncture can regulate the content of neurotransmitters of 5-HT and CA in the brain and spinal cord in rabbits with knee osteoarthritis, which indicates that the two therapies can relieve the injury to the articular cartilage, as well as abates joint pain and stops osteoarthritis development based on the regulation of the synthesis and metabolism of neurotransmitters in the central nervous system.