| 临床大肠埃希氏菌耐药性检测及超广谱β-内酰胺酶流行病学调查 |
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| 引用本文： | 张雪飞,;钟兰兰,;肖胤勃,;方芝莉,;卿芸,;黄盈敏,;冯炼强,;黄曦,;田国宝.临床大肠埃希氏菌耐药性检测及超广谱β-内酰胺酶流行病学调查[J].热带医学杂志,2014(6):721-724. |
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| 作者姓名： | 张雪飞 ;钟兰兰 ;肖胤勃 ;方芝莉 ;卿芸 ;黄盈敏 ;冯炼强 ;黄曦 ;田国宝 |
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| 作者单位： | [1]中山大学中山医学院免疫学教研室,广东广州510080; [2]中山大学热带病防治研究教育部重点实验室,广东广州510080; [3]中山大学人类病毒学研究所,广东广州510080 |
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| 基金项目： | 国家自然科学基金（81201325）; 广东省自然科学基金（102510089010058;S201204006856） |
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| 摘 要： | 目的了解广州地区临床大肠埃希氏菌耐药及超广谱β-内酰胺酶(ESBLs)的流行情况。方法对临床分离鉴定的57株大肠埃希氏菌进行5大类15种抗菌药物的耐药性检测。通过表型确认试验筛选出产ESBLs菌株,采用PCR法检测15种临床重要且常见的耐药基因。结果 57株大肠埃希氏菌对15种抗菌药物产生了不同程度的耐药性。其中有23株产ESBLs,ESBLs产生率为40.4%;24株产TEM,13株产CTX-M(其中,8株产CTX-M-55、4株产CTX-M-14、1株产CTX-M-27),2株产OXA-1。结论广州地区大肠埃希氏菌耐药情况依然严峻。同时,ESBLs流行趋势发生了变化:以前以流行CTX-M-9型为主,现在以CTX-M-1型和CTX-M-9型同时流行为主要特征。
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| 关 键 词： | 大肠埃希氏菌 耐药性 ESBLs 基因型 |
Detection of antibiotic resistance and epidemiological survey of extendedspectrum β-lactamases in clinical Escherichia coli isolates |
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| Institution: | ZHANG Xue-fei, ZHONG Lan-lan, XIAO Yin-bo, FANG Zhi-li, QING Yun, HUANG Ying-min, FENG Lian-qiang, HUANG Xi, TIAN Guo-bao ( 1.Department of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, Guangzhou 510080; 2.Key Laboratory of Tropical Diseases Control, Sun Yat-sen University, Ministry of Education, Guangdong, Guangzhou 510080;3.Institute of Human Virology,Sun Yat-sen University, Guangdong, Guangzhou 510080, China) |
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| Abstract: | Objective To investigate the antibiotic resistance profiles of clinical Escherichia coli isolates from Guangzhou and the epidemiology of ESBLs. Methods Fifty-seven Escherichia coli isolates were underwent susceptibility test of 15antimicrobial agents in 5 major categories and screening tests for ESBLs. ESBLs positive isolates were analyzed for the 15drug-resistance associated genes by PCR. Results Fifty-seven clinical Escherichia coli isolates exhibited different levels of resistance to the tested antimicrobial agents.Twenty-three were confirmed of producing ESBLs,including 24 TEM,13 CTX-M,and 2 OXA-1.Among the 13 CTX-M producers,CTX-M-1 group（8 producing CTX-M-55）and CTX-M-9 group（4 producing CTX-M-14）were the predominant genotypes. Conclusion The drug resistance of clinically isolated Escherichia coli strains in Guangzhou remains challenging and there is a significant change in the prevalence of ESBLs ：CTX-M-1 group and CTX-M-9group have become dominant ESBLs while CTX-M-9 group was the prevailing resistant type in the past. |
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| Keywords: | Escherichia coli antibiotic resistance ESBLs genotype |
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