玻璃化冷冻保存人类早期胚胎的临床应用

目的 探讨玻璃化冷冻技术在人类早期胚胎冻存中的临床应用价值.方法 回顾性分析本中心822个冷冻胚胎复苏周期,依据胚胎冷冻方法的不同分为玻璃化冷冻组(490个周期)和程序化冷冻组(332个周期),比较两组胚胎复苏率、复苏胚胎完整率、胚胎种植率、临床妊娠率等数据.结果 玻璃化冷冻复苏组与程序化冷冻复苏组胚胎复苏率分别为98.8％和82.9％,复苏胚胎完整率分别为96.8％和63.1％,胚胎种植率分别为32.0％和18.1％,临床妊娠率分别为53.9％和33.1％,两组数据比较差异均有统计学意义(P＜0.05).结论 玻璃化冷冻法比程序化冷冻法更适合于人类早期胚胎的冷冻保存.

Clinical application of vitrified early human embryos

Objective To evaluate the clinical application of vitrified human embryos. Methods In the retrospective study, a total of 822 frozen embryos （332 embryos from program freezing and 490 embryos from vitrification freezing） were studied. The rates of embryos survival, blastomere integrity, implantation and clinical pregnancy were compared between the two methods. Results Vitrified embryos had a higher survival rate （98.8% vs 82.9%）, blastomere integrity rate （96.8% vs 63.1%）, implantation rate （32.0% vs 18.1%） and clinical pregnancy rate （53.9% vs 33.1%） then the program frozen embryos. Conclusion Vitrification is an effective method for cryopreservation of human early embryos.