| 应用PCR-SSCP分析我国不同地区常见嗜尸蝇类的研究 |
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| 摘 要： | 目的 探讨应用PCR-SSCP分析我国不同地区常见嗜尸蝇类,揭示其亲源关系及基因差异.方法 采集了中国部分城市,即广州、深圳、阳江、南京、长春、宜昌、北京、武汉、成都等地的常见嗜尸蝇类:家蝇(Muscdomestica),丝光绿蝇(Lucilia sericata),大头金蝇(Chrysomya megacephcda),黑尾麻蝇(Helicophagella melanura),棕尾麻蝇(Boettcherisca peregrina)等.用PCR.SSCP分析我国不同嗜尸蝇类的rDNA-ITS2基因片段.结果 PCR-SSCP分析结果显示,Chrysomya megacephata,Aldrichina grahami(巨尾阿丽蝇),Helicophagella melanura,Muscdomestica,Boettcherisca peregrina rDNA-ITS2基因的单链DNA(ssDNA)迁移率均有明显差异,我国不同地区常见嗜尸蝇种类rDNA-ITS2基因单链DNA迁移图型呈现多态性.结论 我国不同地区常见嗜尸蝇种类的rDNA-ITS2基因存在种内、种间及地理的基因多态性.
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| 关 键 词： | 法医昆虫 嗜尸蝇类 |
Analysis of Different Population of Necrophagous Fly Species from China by PCR-SSCP |
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| Abstract: | Objective To identify the various necrophagous fly species using PCR-SSCP and to analyze the genetic differences of the ITS2 regions. Methods Five carrion fly species,including Musca domestica,Lucilia sericata, Chrysomya megacephala, Helicophagella melanura and Boettcherisca peregrina, were collected from different cities and regions in China. Their rDNA-ITS2 gene was analyzed using PCR-SSCP. Results The electrophoretic mobility rate of ssDNA of rDNA-ITS2 gene of these five species were all different.This observation indicated the genetic polymorphism of the ITS-2. Conclusion This study revealed the genetic diversity and genotype of necrophagous flies collected from different geographic regions. |
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| Keywords: | rDNA-ITS2 PCR-SSCP forensic insects Necrophagous flies rDNA-ITS2 PCR-SSCP |
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