参芪扶正注射液对化疗后小鼠造血功能影响的实验研究

目的 探讨参芪扶正注射液对化疗后小鼠造血功能的影响.方法 用5-氟尿嘧啶（5-FU）225 mg/kg腹腔注射复制骨髓抑制动物模型,治疗组给予参芪扶正注射液5 ml/kg,对照组给予同剂量生理盐水.治疗1周后,做血细胞计数、各系造血祖细胞集落（CFU-GM、CFU-E、CFU-MK）培养和骨髓病理检查.结果 参芪扶正注射液能升高外周血细胞计数：治疗组小鼠外周血的红细胞、白细胞和血小板计数均高于对照组（P〈0.05）.促进造血祖细胞增殖：治疗组小鼠的CFU-GM、CFU-E、CFU-MK集落数均大于对照组（P〈0.05）.改善骨髓抑制：骨髓病理检查对照组出现大片空白区,造血细胞稀少,而治疗组造血组织结构较完整,造血细胞量丰富.结论 化疗后小鼠在骨髓抑制、造血功能低下时,参芪扶正注射液能通过促进骨髓各系造血祖细胞的增殖,改善骨髓造血组织增生,从而促进血细胞的生成.

Effect of Shenqi Fuzheng Injection on the Hematopoietic Function of Mouse after Chemo-Therapy

Objective To explore the effect of Shenqi Fuzheng Injection on the hematopoietic function of mouse after chemo-therapy. Methods 5-FU (225 mg/kg) induced bone marrow depression mouse model was used for the study. Mice of therapeutic group were given Shenqi Fuzheng Injection 5 ml/kg every day and the control ones were injected with saline. After one week, the mice were killed and blood cell count, cultivation of hemopoietic progenitor cells, and pathological examination of the bone marrow were performed. Results Shenqi Fuzheng Injection could increase peripheral blood cell count: The counts of RBC,WBC and PLT in treated mice were higher than in the control ones(P < 0.05). Promote the proliferation of HPC: the counts of CFU-GM, CFU-E and CFU-MK colonies of the treated mice were higher than that in the control ones(P < 0.05).Improve the bone marrow inhibition: pathological examination showed in the control group, the hematopoietic structure was destroyed and there were few hematopoietic cells, while in treated group the structure of bone marrow was rather intact and filled with abundant of hematopoietic cells. Conclusion Shenqi Fuzheng injection can improve proliferation of hematopoietic tissue and blood cells by stimulating growth of hemopoietic progenitor cell in mouse whose bone marrow and hematopoietic function was suppressed after chemotherapy.