聚合酶链反应检测疟疾的研究

目的 建立能鉴别诊断恶性疟原虫间和日疟原虫的PCR检测方法。方法 根据红内期疟原虫SSUr-RNA基因序列，设计合成两对引物，采用PCR技术，检测89份门诊“四热”病人血样，并与镜检法进行比较研究。结果 恶性疟原虫血样能扩增出205bp的特异带，间日疟原虫血样能扩增出120bp的特异带，PCR检测的阳性率为74.16%，镜检法为68.54%,PCR法与镜检法的符合率为94.38%。结论 PCR检测的敏感性和特异性均优于镜检法，对于诊断恶性疟原虫和间日疟原虫以及恶性疟原虫的间日疟原虫的混合感染具有一定的应用价值。

Studies on application of polymerase chain reaction for detecting malaria

Aim To establish a PCR test to detect and differentiate Plasmodiu falciparum and Plasmodium vivax. Methods Two pair of oligonucleotide primers based on the sequence of the SSUrRNA gene was synthesized. With microscopic examination of blood smears as control, PCR was used to detect malaria in outpatient clinics (if China. Results The PCR product of P.falciparum cases was a 205bp fragment and the PCR product of P. vivax cases was a I2Ohp fragment. The positive rate of the PCR test(74. 16%) was significantly higher than that of microscopic examination(68. 54% ). The concordance rate between these two diagnostic methods was 94. 38%. Conclusions Ttie PCR test is specific, sensitive and has a great potential in identifying different Plasmodium species and detecting mixed infection.