| 弓形虫MIC8基因对弓形虫病的免疫保护性研究 |
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| 引用本文： | 赵焕阁,谭光宏,黄凤迎,王华,郭峻莉,黄用豪.弓形虫MIC8基因对弓形虫病的免疫保护性研究[J].热带医学杂志,2011,11(11):1252-1254,1275. |
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| 作者姓名： | 赵焕阁 谭光宏 黄凤迎 王华 郭峻莉 黄用豪 |
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| 作者单位： | 海南医学院海南省热带病重点实验室,海南海口,571101 |
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| 基金项目： | 海南省自然科学基金(809018) |
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| 摘 要： | 目的观察弓形虫MIC8基因对弓形虫病免疫保护性的效果。方法用PCR方法扩增弓形虫MIC8基因,定向克隆到真核表达质粒pEGFP-N1中,构建重组表达载体pEGFP-MIC8;MIC8-EGFP转染293T细胞,荧光显微镜观察表达情况;然后分别用质粒pEGFP-MIC8、pEGFP-N1空载体及生理盐水肌肉注射免疫BALB/c小鼠,共免疫3次,末次免疫3周后用弓形虫RH株速殖子感染免疫小鼠。结果 PCR扩增弓形虫MIC基因序列正确,构建的重组表达载体pEGFP-MIC8鉴定正确,荧光显微镜下观察到重组质粒能够在293T细胞中表达。弓形虫攻击感染后,质粒pEGFP-MIC8免疫小鼠与对照组相比,小鼠的存活时间有一定的延长。结论构建的真核表达重组质粒pEGFP-MIC8对抗击弓形虫攻击感染能够激发一定的免疫保护性。
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| 关 键 词： | 弓形虫 MIC8 免疫保护 |
Toxoplasma gondii MIC8 gene has immunoprotective effect on toxoplasmosis |
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| ZHAO Huan-ge,TAN Guang-hong,HUANG Feng-ying,WANG Hua,GUO Jun-li,HUANG Yong-hao.Toxoplasma gondii MIC8 gene has immunoprotective effect on toxoplasmosis[J].Journal Of Tropical Medicine,2011,11(11):1252-1254,1275. |
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| Authors: | ZHAO Huan-ge TAN Guang-hong HUANG Feng-ying WANG Hua GUO Jun-li HUANG Yong-hao |
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| Institution: | ZHAO Huan-ge,TAN Guang-hong,HUANG Feng-ying,WANG Hua,GUO Jun-li,HUANG Yong-hao(Key Laboratory for Tropical Diseases,Hainan Medical College,Hainan,Haikou 571101,China) |
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| Abstract: | Objective Investigate immunoprotective effects of T.gondii microneme 8(MIC8) on toxoplasmosis.Methods MIC8 gene fragment was amplified by PCR and cloned into pEGFP-N1 to generate eukaryotic expression construct pEGFP-MIC8;GFP tagged MIC8 protein was expressed in the 293 cells by transfection and observed using a fluorescence microscope;Plasmid pEGFP-MIC8,pEGFP-N1 and NS were injected into BALB/c mice muscle three times.Each injected mouse was challenged with tachyzoites of T.gondii after the last immunizati... |
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| Keywords: | Toxoplasma gondii microneme 8 immuno-protection |
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