Livin、Survivin蛋白在非小细胞肺癌胞核中的表达及意义

目的研究凋亡抑制蛋白Survivin和Livin在tPA,细胞肺癌（NSCLC）细胞核中阳性表达（核阳）水平及其临床意义。方法采用免疫组织化学sP法检测40例NSCLC组织和20例癌旁正常肺组织中Survivin蛋白和Livin蛋白核阳水平。并分析其与临床特征的关系。结果在NSCLC和癌旁正常肺组织中，Survivin蛋白细胞核表达的阳性率分别为80％（32／40）和5％（1／20），Livin蛋白细胞核表达的阳性率分别为80％（32／40）和10％（2／20）。Survivin蛋白和Livin蛋白核阳在NSCLC组织中的表达显著高于癌旁组织（P〈0．05）。Survivin蛋白核阳与淋巴结转移、肿瘤分期高度相关（尸〈0．05）。结论Survivin蛋白和Livin蛋白在NSCLC细胞核中高表达，可能成为NSCLC诊断的新指标和治疗的潜在靶点。

The Nuclear Staining of Survivin and Livin and Its Clinical Significance in Non-Small Cell Lung Cancer

Objective In this study,we will investigate the nuclear staining of Survivin and Livin and its clinical significance in non-small cell lung cancer （NSCLC）. Methods 40 Paraffin-embedded NSCLC tissue blocks obtain randomly and 20 Paraffln-embedded bullae of lung tissue blocks were obtained for immunohistochemical staining to evaluate the expression of Survivin and Livin. The Chi-Square test was performed to evaluate the correlation of staining and clinical outcome. Results The nuclear positive rates of Survivin in NSCLC and bullae of lung tissue were 80% （32/40） and 5% （1/20）, respectively. The nuclear positive rates of Livin in NSCLC and bullae of lung tissue were 80% （32/40） and 10% （2/20）, respectively. The nuclear staining of Survivin and Livin were higher in NSCLC tissue samples than that in bullae of lung samples （P〈0.05）. The nuclear staining of Survivin was associate with lymph node metastasis and stage of tumor （P〈0.05）. Conclusions The expressions of Survivin and Livin were high in NSCLC. It will provide a new target for the diagnosis and treatment of lung carcinoma.