肺气虚证和肺阳虚证模型大鼠血清中细胞因子含量变化研究

目的：探讨肺气虚和肺阳虚证大鼠血清中肿瘤坏死因子α（tumor necrosis factor-α,TNF-α）、白细胞介素6（interleukin-6,IL-6）和白细胞介素8（interleukin-8,IL-8）的变化及其作用。方法：雌雄Wistar大鼠30只,随机分为对照组、肺气虚证组、肺阳虚证组,各组10只,其中肺气虚证组以单纯烟熏造模（香烟＋锯末、刨花,0.5h/d）,肺阳虚证组采用烟熏＋寒冷刺激造模（0～2℃,2h×2次/d）,以上各组连续处理7周后取大鼠腹主动脉血,采用放射免疫法测定血清中TNF-α、IL-6、IL-8含量。结果：对照组始终无症状,肺气虚证、肺阳虚证组大鼠均出现咳嗽、痰涎清稀,且肺阳虚证组大鼠出现少动、大便稀薄等寒象;两模型大鼠血清中细胞因子TNF-α、IL-8的含量均高于对照组（P〈0.05）,且肺阳虚证组更为明显;气虚证组IL-6含量显著高于对照组（P〈0.01）,但肺阳虚证组IL-6含量的升高较对照组无统计学差异（P〉0.05）。结论：细胞因子TNF-α、IL-6、IL-8参与了肺气虚证与肺阳虚证的病理发展过程。

Study on the Levels of Serum Cytokines in Lung Qi and Yang Deficiency Syndrome Rats

Objective：To investigate the serum cytokine tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）and interleukin-8（IL-8）in Lung Yang Deficiency and Lung Qi Deficiency Syndrome rats.Methods：30 male and female Wister rats were randomly divided into control group,the Lung Qi Deficiency Syndrome group and Lung Yang Deficiency Syndrome group,10 in each group,in which Lung Qi Deficiency group rats were smoked only（including cigarettes,sawdust and wood shavings,0.5 / day）,Lung Yang deficiency group rats were treated by smoke and cold stimulus（0～2℃,2 hours×2 times / day）.By the end of 7th weeks,abdominal aorta blood of rats were drawn and the serum TNF-α,IL-6 and IL-8 content were detected by radioimmunoassay.Results：The control group rats had no symptoms,but the two model rats coughed and expectorated,and Lung Yang Deficiency group rats appeared less activity,dilute stool.Serum cytokine TNF-α,IL-8 levels were higher in the two model groups compared with the control（P〈0.05）;IL-6 level in Lung Qi deficiency syndrome group were significantly higher（P〈0.01）than the control,but there was no significant difference（P〉0.05）between the Lung Yang Deficiency and the control.Conclusion：Cytokine TNF-α,IL-6 and IL-8 involved in the pathological development of Lung Yang Deficiency and Lung Qi deficiency.