ACE基因的插入/缺失多态性与2型糖尿病伴高血压的相互关系

目的探讨血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性与2型糖尿病伴高血压之间的相互关系。方法以ACE基因为候选基因,应用PCR方法,检测用例2型糖尿病伴高血压患者及117例2型糖尿病无高血压患者的ACE基因。结果2组研究对象DD、DⅠ、Ⅱ基因型分布频率分别为27.16％vs 20.51％、41.98％vs 41.88％、30.86％vs37.61％,D、I等位基因携带率分别为48.15％vs41.45％、51.85％vs58.55％,均有显著性差异（P<0.05）,提示ACE基因DD型及D等位基因与2型糖尿病并发高血压的发生有相关性。糖尿病患者中ACE基因I/D多态呈DD型者发生高血压的风险率为Ⅱ型者的1.32倍,且糖尿病伴高血压患者病程显著延长。正常血压糖尿病患者ACE基因 I/D多态性与单纯收缩压（SBP）升高患者ACE基因I/D多态性之间无显著差异,而与单纯舒张压（DBP）升高患者ACE基因I/D多态性之间显著相关。结论ACE基因I/D多态性参与2型糖尿病伴高血压的发病,D等位基因可能是2型糖尿病并发高血压的一个危险因子,且DBP升高在糖尿病伴高血压的发生中起重要作用。

Association of insertion/deletion polymorphism in angiotensin-converting enzyme gene with hypertensive type 2 diabetes mellitus

Objective To elucidate the interaction between the insertion/deletion (I/D) polymorphism in angiotensin converting enzyme (ACE) gene and hypertensive type 2 diabetes mellitus. Method I/D polymorphism in ACE gene was determined with PCR technique in 82 hypertensive and 117 non-hypertensive patients with type 2 diabetes. Results The comparison between the 2 groups revealed a significant difference in DD/DI/II genotype distribution and DA allele carrying (x2=6.41, PO.05), indicating an association of DD genotype and D allele carrying with the occurrence of hypertensive type 2 diabetes. Diabetic patients with I/D polymorphism in ACE gene complicated by hypertension had 1.32 times higher risk than non-hypertentive type 2 diabetic patients, and the course of hypertentive type 2 diabetes was significantly lengthened. No significant difference of I/D polymorphism in ACE gene was identified between normotensive diabetic patients and diabetic patients with elevated diastolic blood pressure, but the close correlation was found between normotensive diabetic patients and diabetic patients with elevated systolic pressure. Conclusions I/D polymorphism in ACE gene contributed to development of hypertentive type 2 diabetes. D allele might be an important risk factor for hypertentive type 2 diabetes, and elevation of diastolic blood pressure plays an important role in the occurrence of hypertentive type 2 diabetes.