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| 两种大鼠心肌梗死模型的比较研究 | |
| 作者姓名： | Tong M He L Liu WL Zhou SH |
| 作者单位： | 1. 中南大学湘雅二医院动物实验室,湖南长沙,410012 2. 中南大学湘雅二医院麻醉科,湖南长沙,410012 3. 中南大学湘雅二医院胸外科,湖南长沙,410012 4. 中南大学湘雅二医院心内科,湖南长沙,410012 |
| 基金项目： | 湖南省科研条件创新专项计划(2010TD2015) |
| 摘 要： | 目的通过对大鼠冠状动脉前降支结扎法和超声波凝固法制作心肌梗死模型进行对比研究,探讨两种心梗模型的优缺点。方法 45只SD大鼠,随机分成假手术组(n=15)、冠状动脉结扎组(n=15)、超声刀组(n=15)。假手术组:冠脉左前降支只套线,不结扎;冠状动脉结扎组:用620缝合线结扎大鼠左冠状动脉前降支;超声刀组:用超声刀凝固冠状动脉左前降支。术后4周分别测定大鼠血清肌酸磷酸激酶(CK)及其同功酶(CK-MB),乳酸脱氢酶(LDH),天门冬氨酸氨基转换酶(AST)水平,并行心肌HE染色进行病理组织学分析,探讨两种模型的优劣。结果与冠脉结扎组比较,超声刀组血清CK、CK-MB、LDH、AST活性均无明显差异(P>0.05);HE染色显示:两组心肌组织均出现凝固性梗死,炎细胞浸润,继而有肉芽组织长入,最后形成瘢痕。冠状动脉结扎组大鼠术后死亡率50%,超声刀组大鼠术后死亡率20%,差异有统计学意义(P<0.05)。结论二者均可有效复制心肌梗死模型。超声刀凝固法操作简单,死亡率低,能建立稳定的大鼠心肌梗死模型,可用于心肌梗死的相关研究。 |
| 关 键 词： | 冠状动脉结扎法 超声刀 心肌梗死 |
Comparison of two rat models of myocardial infarction |
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| Tong M,He L,Liu WL,Zhou SH.Comparison of two rat models of myocardial infarction[J].Journal of Southern Medical University,2011,31(11):1941-1943. | |
| Authors: | Tong Min He Liang Liu Wen-liang Zhou Sheng-hua |
| Institution: | TONG Min1,HE Liang2,LIU Wen-liang3,ZHOU Sheng-hua4 1Department of Animal Center,2Department of Anesthesiology,3Department of Thoracic Surgery,4Department of Cardiology,The Second Xiangya Hospital of the Central South University,Changsha 410012,China |
| Abstract: | Objective To compare two rat models of myocardial infarction established by coronary artery ligation and harmonic scalpel method for their advantages,disadvantages and applications.Methods Sixty Sprague Dawley rats were randomly divided into sham-operated group,coronary artery ligation group and harmonic scalpel group.In the latter two groups,myocardial infarction was induced by ligation of the left anterior descending coronary artery and coagulation of the artery,respectively.Four weeks after the operation... |
| Keywords: | coronary artery ligation method myocardial infarction harmonic scalpel |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |