机械法准分子激光上皮瓣下角膜磨镶术后早期泪液中肝细胞生长因子含量与角膜上皮下雾状混浊的相关性

目的 观察兔眼机械法准分子激光上皮瓣下角膜磨镶术(Epi-LASIK)术后早期泪液中肝细胞生长因子(HGF)含量的动态变化及其与角膜上皮下雾状混浊(雾状混浊)的关系.方法 24只(48眼)新西兰白兔,双眼分别建立切削深度100μm Epi-LASIK和切削深度150μm Epi-LASIK动物模型.采用酶联免疫反应吸附实验双抗体夹心法测定两组术前、术后3、7、14、30 d泪液中HGF的水平,并观察两组术后雾状混浊的情况.结果 不同切削深度两组Epi-LASIK术后角膜上皮均于3~5 d愈合.两组均于术后3 d开始形成雾状混浊,伴随泪液中HGF含量的升高.150μm切削深度组术后3、7、14、30 d雾状混浊程度较100μm组明显(P<0.05),且泪液中HGF的水平明显高于100μm组(P<0.05).结论 两组Epi-LASIK术后早期泪液中HGF与雾状混浊的程度表现为正相关,且与切削深度相关.

Association between hepatocyte growth factor in tears and corneal haze in rabbits early after epipolis laser in situ keratomileusis

Objective To study the dynamic changes of levels of hepatocyte growth factor (HGF) in tears and their association with corneal haze in rabbits early after epipolis laser in situ keratomileusis (Epi-LASIK). Methods Twenty-four New Zealand rabbits received Epi-LASIK with an ablation depth of 100 μm in one eye and of 150 μm in the other eye. Before and at 3, 7, 14, and 30 days after the surgery, the level of HGF in tears collected from the rabbits was measured using enzyme-linked immunosorbent assay (ELISA), and corneal haze was graded after surgery. Results In all the rabbits, corneal epithelium healing occurred in 3 to 5 days after Epi-LASIK. Corneal haze appeared 3 days postoperatively in the rabbits accompanied by increased levels of HGF in tears. At 3, 7, 14, and 30 days after the surgery, the rabbits with an ablation depth of 150 μm showed more obvious corneal haze (P<0.05) and significantly higher levels of HGF in tears than those with an ablation depth of 100 μm (P<0.05). Conclusion In rabbits receiving Epi-LASIK, HGF levels in tears and the grade of corneal haze show a positive correlation early after the surgery and are both related with the depth of ablation.