热疗与阿霉素联合作用动物的实验研究

目的 研究阿霉素与热疗联合作用对人B细胞淋巴瘤细胞株Raji裸鼠成瘤及肿瘤生长的影响.方法 将24只BALB/C裸鼠分为4组:对照组(37℃)、化疗组(37 ℃ ADM)、热疗组(42℃)、热化疗组(42℃ ADM),每组6只.将对数生长期的Raji细胞配制成每0.2ml含5×106个Raji细胞的悬液,每只裸鼠皮下接种5×106的Raji细胞,观察各组裸鼠成瘤时间、成瘤率、肿瘤体积变化.并绘制肿瘤生长曲线.成瘤后3周,处死老鼠,取肿块称质量,计算抑瘤率,肿瘤组织行常规病理检查.结果 对照组(37℃)、化疗组(37℃ ADM)、热疗组(42℃)、热化疗组(42℃ ADM)的肿瘤出现时间分别为(11.2±1.7)、(20.2±2.3)、(15.3±1.6)及(23.8±1.7)d,热化疗组成瘤时间明显延长(P＜0.01).热化疗能明显减缓Raji细胞BALB/C裸鼠体内肿瘤的生长速度.治疗结束,热化疗组的肿块较对照组肿块明显缩小.对照组(37℃)、化疗组(37℃ ADM)、热疗组(42℃)、热化疗组(42 ℃ ADM)裸鼠的肿瘤质量分别为(3.33±0.57)、(2.26±0.28)、(2.76±0.26)及(1.73±0.33)g,热化疗组抑瘤率为48.0%.结论 热联合化疗能有效抑制肿瘤的生长,为恶性淋巴瘤的治疗提供了另一种辅助治疗方法.

Adriamycin combined with hyperthemia inhibits transplanted B cell lymphoma in nude mice

OBJECTIVE: To study the effect of adriamycin combined with hyperthermia on tumor formation and growth of human B lymphoma cell line (Raji) in nude mice. METHODS: Twenty-four BALB/C nude mice were divided into control group (37 degrees celsius;), chemotherapy group (37 degrees celsius;+ADM), hyperthermia group (42 degrees celsius;) and thermochemotherapy group (42 degrees celsius;+ADM), and in each mouse, 5 x 10(6) Raji cells were injected subcutaneously. The time and rate of tumor formation were observed, the tumor diameter was measured every 3 days and the tumor volume calculated to obtain the growth curves of the tumors. Three weeks after tumor formation, all the mice were executed to observe the histopathological changes of the tumor with HE staining. RESULTS: The time of tumor formation in the control, chemotherapy, hyperthermia and thermochemotherapy groups were 11.2-/+1.7, 20.2-/+2.3, 15.3-/+1.6 and 23.8-/+1.7 days, respectively. Three weeks after tumor formation, the average weight of the tumors were 3.33-/+0.57, 2.26-/+0.28, 2.76-/+0. 26 and 1.73-/+0. 33 g, respectively, and the tumor growth inhibition rate was 48.0% in the thermochemotherapy group. CONCLUSION: Thermochemotherapy can be effective in inhibiting tumor growth and provides an alternative of adjuvant therapy for malignant B cell lymphoma.