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PCR扩增IgH和TCR_β基因重排在检测淋巴细胞白血病微小残留病中的应用
引用本文:赵洪宁,黄志光,朱梅刚,张素娟,董敬朋.PCR扩增IgH和TCR_β基因重排在检测淋巴细胞白血病微小残留病中的应用[J].南方医科大学学报,1995(2).
作者姓名:赵洪宁  黄志光  朱梅刚  张素娟  董敬朋
作者单位:南方医院小儿科,济南军区医学高等专科学校
摘    要:利用IgH和TCR_β克隆性基因重排原理,用多聚酶链反应(PolymeraseChainReaction.PCR)技术检测小儿急性淋巴细胞白血病(ALL)骨髓和外周血共13例。结果:ALL初治的2例患儿PCR检测全部呈现阳性特异性单带。ALL完全缓解(CR)的11例患儿中阳性7例,阴性4例,其中2例骨髓移植(BMT)患儿移植前PCR阳性,移植后转为阴性。1例自体骨髓移植(auto-BMT)患儿于移植后2个月时PCR检测又重新出现阳性,但临床无复发现象。4例对照者PCR均为阴性。结果表明PCR技术具有简便、快速、敏感、特异性强等优点,在检测微小残留(MRD)中具有广阔前景。

关 键 词:急性淋巴细胞白血病  微小残留病  基因重排  多聚酶链反应

Application of PCR in detection of IgH and TCR gene rearrangement in MRD
Zhao Hongning,Huang Zhiguang,Zhu Meigang Zhang Sujuan,Dong Jingpeng.Application of PCR in detection of IgH and TCR gene rearrangement in MRD[J].Journal of Southern Medical University,1995(2).
Authors:Zhao Hongning  Huang Zhiguang  Zhu Meigang Zhang Sujuan  Dong Jingpeng
Institution:Zhao Hongning,Huang Zhiguang,Zhu Meigang Zhang Sujuan,Dong Jingpeng Department of pediatrics,Nanfang Hospital Department of Pathology
Abstract:PCR technique was used to detect clonal rearrangement of IgH and TCR genes in DNA samples frombone marrow and peripheral blood of 13 children suffering from acute lymphoblastic leukemia (ALL).Apositive specific single band was observed in 2 patients of ALL without treatment. 7 out of 11 ALL in com-plete remission(CR)stage were also positive and 4 cases were negetive. 2 out of these 11 cases were previ-ously positive before treatment by bone marrow transplantation(BMT),and 1 case showed positive resultafter two months of auto-BMT without any symptom of recurrence. ALL 4 controls were negetive. Ourresults indicate that the PCR technique is simple,fast,sensitive and highly specific. It is very useful in thedetection of minimal residual disease(MRD).
Keywords:acute lymphoblastic leukemia  minimal residual disease  gene    rearrangement  polymerase Chain reaction(PCR)
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