| 二甲双胍对人结肠癌细胞株SW-480增殖的影响 |
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| 引用本文： | 周小智,薛耀明,朱波,沙建平.二甲双胍对人结肠癌细胞株SW-480增殖的影响[J].南方医科大学学报,2010,30(8). |
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| 作者姓名： | 周小智 薛耀明 朱波 沙建平 |
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| 作者单位： | 南方医科大学南方医院内分泌代谢科,广东,广州,510515 |
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| 摘 要： | 目的 观察二甲双胍对人结肠癌细胞株SW-480增殖的影响并初步探讨其可能机制.方法 不同浓度的二甲双胍干预结肠癌细胞株SW-480,MTT法比较其生长曲线的变化,采用流式细胞术研究二甲双胍对SW-480细胞周期的影响,Western blotting测定CyclinD1的表达,用TRAP银染法观察其对端粒酶活性的影响.结果 MTT结果示二甲双胍可以抑制人结肠癌细胞的增殖,并呈时间浓度依赖性.PBS对照组与5 mmol/L二甲双胍干预组72 h后的细胞G0/G1期所占百分比分别为55.81±0.63,63.38±0.99;S期所占百分比分别为31.11±3.05,25.29±1.64;G2/M期所占百分比分别为13.09±3.00,11.33±2.60.Western blotting示5 mmol/L二甲双胍干预组72 h后CyclinD1的表达较PBS对照组明显减弱.TRAP银染法检测示5 mmol/L二甲双胍干预SW-480细胞72 h后可以显著抑制端粒酶活性.结论 二甲双胍能抑制人结肠癌细胞的增殖,其主要机制可能与G0/G1期细胞周期阻滞、下调CyclinD1的表达及抑制端粒酶活性有关.
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| 关 键 词： | 二甲双胍 SW-480细胞 细胞周期 端粒酶活性 |
Effects of metformin on proliferation of human colon carcinoma cell line SW-480 |
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| ZHOU Xiao-zhi,XUE Yao-ming,ZHU Bo,SHA Jian-ping.Effects of metformin on proliferation of human colon carcinoma cell line SW-480[J].Journal of Southern Medical University,2010,30(8). |
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| Authors: | ZHOU Xiao-zhi XUE Yao-ming ZHU Bo SHA Jian-ping |
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| Institution: | ZHOU Xiao-zhi,XUE Yao-ming,ZHU Bo,SHA Jian-ping Department of Endocrinology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To observe the effect of metformin on the proliferation of SW-480 cells and study the possible mechanism. Methods The proliferation of SW-480 cells treated with different concentrations of metformin was assessed by MTT assay, and the cell cycle changes were analyzed by flow cytometry. The expression of cyclin D1 in the treated cells was detected by Western blotting, and telomerase activity examined by telomeric repeat amplification protocol (TRAP) sliver staining. Results Metformin decreased the p... |
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| Keywords: | CyclinD1 |
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