蛋白酶体抑制剂MG-132在大鼠重症急性胰腺炎及其肺损伤中的保护作用

目的观察蛋白酶体抑制剂MG-132在大鼠重症急性胰腺炎（SAP）中的保护作用，并探讨其可能机制。方法SD大鼠30只，5％牛磺胆酸钠逆行胆胰管注射制作重症急性胰腺炎（SAP）模型，观察各组大鼠胰腺、肺组织形态学改变，并测定血清淀粉酶及胰腺、肺组织髓过氧化物酶（MPO）活性。结果MG-132治疗组与胰腺炎组及假手术组比较，血清淀粉酶、胰腺及肺组织MPO活性下降（P〈0．05），组织病理学改变均有不同程度的改善（P〈0．05）。结论制模前30min腹腔注射10mg／kg的MG-132可以明显减轻5％牛磺胆酸钠诱导的大鼠的肺损伤严重程度，并可减轻胰腺炎所致的肺损伤。

Protective effect of proteasome inhibitor MG-132 in rats with severe acute pancreatitis and lung injury

Objective To observe the protective effect of the proteasome inhibitor MG-132 in rats with severe acute pancreatitis (SAP) and the associated lung injury. Methods In rat models of the SAP established with injection of 5% sodium taurocholate into the biliary-pancreatic duct, the changes of the serum amylase and myeloperoxidase (MPO) activity in the pancreatic and lung tissues were evaluated. The pathological changes of the pancreatic and lung tissues were also observed. Results MG-132 significantly decreased serum amylase, pancreatic weight/body weight ratio, and pancreatic and pulmonary myeloperoxidase activity (P<0.05). Histopathological examinations revealed milder edema, cellular damage, and inflammation in the pancreatic and lung tissues of rats pretreated with the peptide (P<0.05). Conclusion MG-132 ameliorates SAP and the associated lung injury in rats.