| K~+对三链DNA形成的影响 |
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| 引用本文： | 刘定燮!广州市,王昌才!广州市.K~+对三链DNA形成的影响[J].南方医科大学学报,1997(4). |
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| 作者姓名： | 刘定燮!广州市 王昌才!广州市 |
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| 作者单位： | Institute of Molecular Biology，The First Military Medical University of PLA，GuangZhou，510515 |
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| 摘 要： | 用电泳迁移分析方法及DNaseⅠ足迹实验研究了脱氧寡核苷酸与乙肝病毒（HBV）核衣壳启动子（Cp）片段形成三链DNA中的K~＋效应。K~＋对三链DNA形成的抑制作用大小取决于不同的脱氧寡核苷酸。碱基的错配可使K~＋的抑制作用显著增强，提示K~＋的效应强弱可能与三链结构中碱基准积的连续程度有关。因此反应体系中K~＋的存在能提高三链DNA形成的特异性。
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| 关 键 词： | 三链DNA 脱氧寡核苷酸 钾离子 |
Effects of K~+ on triplex DNA formation |
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| Liu Dingxie, Wang Changcai.Effects of K~+ on triplex DNA formation[J].Journal of Southern Medical University,1997(4). |
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| Authors: | Liu Dingxie Wang Changcai |
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| Abstract: | The effects of K+ on the triplex-formation between oligodeoxyribonucleotides and core promoter(Cp) fragment of hepatitis B virus (HBV) were investigated by electrophoretic mobility shift analysis andDNase I footprinting. The inhibitory effects of K+ were determined by different oligodeoxyribontlcleotides.Base mismatching could enhance the inhibitory effects of K+,suggesting that the effects of K+,were inter-related with the succession of base stacking in triplex DNA. In conclusion,the existence of K+ in reactionbuffer may increase the speciflcity of triplex-formation. |
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| Keywords: | Triplex DNA Oligodeoxyribonucleotide Potassium |
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