巨细胞病毒感染对重型β-地中海贫血儿童异基因造血干细胞移植后早期T细胞亚群的影响

目的探讨巨细胞病毒(CMV)对儿童重型β-地中海贫血(地贫)儿童异基因造血干细胞移植(Allo-HSCT)后180 d外周血T细胞亚群的变化。方法对我院儿科造血干细胞移植中心2010年1月～2011年1月儿童重型地贫Allo-HSCT 35例病例半年后的T细胞亚群进行分析,比较有CMV(13例)和无CMV感染组(22例)在造血干细胞移植后早期T细胞的变化。结果半年内有CMV组CD8+比率高于无CMV感染组,但CD4+比率低。在CMV感染组伴随CD8+CD28+细胞比率降低,CD8+CD28-细胞比率和CD8+比率升高,同时发现CD8+与CD8+CD28-细胞比率呈线性相关性。结论重型β-地中海贫血儿童在异基因造血干细胞移植后,CMV感染后会导致CD8+CD28-细胞堆积,是构成CD8+细胞升高的主因,且两者比率呈线性相关。

Impact of cytomegalovirus infections on T lymphocyte subsets in children with β-thalassemia major early after allogeneic hematopoietic stem cell transplantation

Objective To investigate the effect of human cytomegalovirus(HCMV) infection on T lymphocyte subsets in children with β-thalassemia major(TM) during the initial 6 months after allogeneic hematopoietic stem cell transplantation(Allo-HSCT).Methods From January,2010 to January,2011,35 children with TM underwent Allo-HSCT.Peripheral blood samples were obtained from the children 6 month after the transplantation to examine the changes of T lymphocytes subsets in relation to HCMV seropositivity.Results Thirteen children were found seropositive and 22 were seronegative for HCMV.The HCMV-seropositive children had a higher CD8+ cell percentage but a lower CD4+ cell percentage than those without HCMV infection.Compared with those seronegative for HCMV,the children with HCMV seropositivity showed increased percentages of CD8+ cells and CD8+CD28-cells with a decreased percentage of CD8+CD28+ cells.A positive linear correlation was found between the percentages of CD8+CD28-cells and CD8+ cells.Conclusion HCMV infection can lead to the accumulation of CD8+CD28 cells to cause increased CD8+ T cells in the peripheral blood in TM children after Allo-HSCT.The percentages of CD8+CD28-cells has a positive linear correlation to that of CD8+ cells.