Mir-106b表达与食管鳞状细胞癌的关系

目的 研究微小RNA106b(mir-106b)在食管鳞状细胞癌组织中的表达情况,并进一步分析其与肿瘤的临床病理学特征及预后的相关性.方法 收集200例2001～2007年中国医学科学院接收的食管鳞状细胞癌患者手术切除获得的肿瘤组织和相对应的癌旁组织,采用qRT-PCR方法检测mir-106b在组织中的相对表达水平,并通过northern blot进一步验证其表达情况.应用统计学方法分析mir-106b表达量与肿瘤临床病理学特征及预后的相关性.结果 相对于癌旁组织,食管鳞状细胞癌肿瘤组织中mir-106b的表达水平显著升高,统计学数据显示,mir-106b的表达量与淋巴结转移、肿瘤分期及吸烟有相关性(P＜0.05).并且,mir-106b表达水平较低的患者的生存率(60个月)显著高于mir-106b表达水平较高的患者(37个月,P=0.024).另外,Cox回归分析显示,mir-106b的表达量、区域淋巴结转移及吸烟均为是食管鳞状细胞癌患者独立预后因子(P＜0.05).结论 mir-106b在食管鳞状细胞癌患者肿瘤组织中高表达,且与淋巴结转移及预后不良密切相关,可用作ESCC的诊断及预后生物标志物.

Expression of mir-106b in esophageal squamous cell carcinoma

Objective To investigate the expression of mir-106b in esophageal squamous cell carcinoma (ESCC) tissue and analyze its correlation with the cilnicopathologic features of ESCC.Methods A total of 200 fresh surgical specimens of ESCC and adjacent tissues collected between 2001 and 2007 were examined for expressions of mir-106b using real-time PCR (RT-PCR).Northern blot analysis for mir-106b was performed in 4 pairs of samples to confirm the RT-PCR results.The relationship between mir-106b expression and clinicopathological features and prognosis of the patients were analyzed.Results Mir-106b was expressed at significantly higher levels in ESCC tissues than in the paired adjacent tissues.Overexpression of mir-106b was associated with lymph node metastasis,stage of TNM classification and smoking (P＜0.05).The survival rate of patients with low mir-106b expression was higher than that of patients with high mir-106b expression (60 vs 37 months,P=0.024).Cox regression analysis indicated that the expression of mir-106b,lymph node metastasis and smoking were independent prognostic factors for ESCC (P＜0.05).Conclusion Mir-106b is overexpressed in ESCC tumors,and its overexpression is strongly associated with lymph node metastasis and a poor prognosis.Mir-106b expression is an independent prognostic factor for ESCC and can serve as a biomarker for diagnosis and prognostic evaluation of ESCC.