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Smac在顺铂促非小细胞肺癌细胞株凋亡中的作用
引用本文:成先东,蔡绍曦,赵海金,李文军,佟万成.Smac在顺铂促非小细胞肺癌细胞株凋亡中的作用[J].南方医科大学学报,2008,28(3):389-391.
作者姓名:成先东  蔡绍曦  赵海金  李文军  佟万成
作者单位:1. 南方医科大学,南方医院呼吸科,广东,广州,510515
2. 南方医科大学,公共卫生与热带医学学院,广东,广州,510515
摘    要:目的 探讨Smac在顺铂诱导非小细胞肺癌(NSCLC)细胞株凋亡中的作用.方法 不同浓度顺铂刺激NSCLC细胞株A549,四甲基偶氮唑蓝法检测细胞增殖,Annexin V/P双染流式细胞仪检测细胞凋亡,RT-PCR、Western blotting检测Smac mRNA及蛋白表达.结果 顺铂对A549细胞株具有增殖抑制作用,且呈浓度依赖性.顺铂对A549细胞株具有促凋亡作用,且呈浓度依赖性.顺铂作用后,Smac的mRNA及蛋白表达增加,且呈浓度依赖性.结论 smac在顺铂诱导NSCLC细胞株A549凋亡过程中,可能起到促凋亡的作用.

关 键 词:顺铂  非小细胞肺癌  细胞凋亡  Smac  Smac  顺铂诱导  非小细胞肺癌  细胞株凋亡  促凋亡作用  lung  cancer  cells  apoptosis  凋亡过程  浓度依赖性  增殖抑制作用  结果  蛋白表达  mRNA  Western  blotting  细胞凋亡  流式细胞仪检测  双染  Annexin  细胞增殖
文章编号:1673-4254(2008)03-0389-03
修稿时间:2007年12月6日

Role of Smac in cisplatin-induced apoptosis of non-small cell lung cancer cells
CHENG Xian-dong,CAI Shao-xi,ZHAO Hai-jin,LI Wen-jun,TONG Wan-cheng.Role of Smac in cisplatin-induced apoptosis of non-small cell lung cancer cells[J].Journal of Southern Medical University,2008,28(3):389-391.
Authors:CHENG Xian-dong  CAI Shao-xi  ZHAO Hai-jin  LI Wen-jun  TONG Wan-cheng
Institution:Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. pcwarmfamily@126.com
Abstract:OBJECTIVE: To investigate the role of Smac in cisplatin-induced apoptosis of non-small lung cancer cells in vitro. METHODS: Non-small cell lung cancer A549 cells were incubated in the presence of cisplatin at different concentrations, and the cell proliferation status was observed using MTT assay. Flow cytometry was used for evaluation of the apoptosis of the incubated cells, and the expressions of Smac mRNA and protein were detected by RT-PCR and Western blotting, respectively. RESULTS: Cisplatin inhibited the proliferation and induced apoptosis of A549 cells both in a concentration-dependent manner. Cisplatin also increased the expression of Smac at both the mRNA and protein levels, which was also concentration-dependent. CONCLUSION: Increased Smac expression may play a critical role in cisplatin-induced apoptosis of the non-small cell lung cancer cells in vitro.
Keywords:cisplatin  non small cell lung cancer  apoptosis  Smac  
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