超声造影评价右美托咪定对兔肾缺血/再灌注损伤时微循环灌注的影响 |
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引用本文: | 斯妍娜,韩流,张媛,陈利海,徐亚杰,孙凡,潘笑笑,曾令清,鲍红光.超声造影评价右美托咪定对兔肾缺血/再灌注损伤时微循环灌注的影响[J].南方医科大学学报,2016(5):628-632. |
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作者姓名: | 斯妍娜 韩流 张媛 陈利海 徐亚杰 孙凡 潘笑笑 曾令清 鲍红光 |
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作者单位: | 南京医科大学附属南京医院 南京市第一医院 麻醉科,江苏 南京,210006 |
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基金项目: | 国家自然科学基金(81401620);南京市医学科技发展项目基金(YKK12085) Supported by National Natural Science Foundation of China (81401620) |
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摘 要: | 目的:应用超声造影定量分析技术评价右美托咪定(DEX)对兔肾缺血/再灌注(I/R)损伤时微循环灌注的影响。方法新西兰家兔24只随机分成3组(每组8只):对照组、肾缺血/再灌注损伤组(I/R组)和右美托咪定组(DEX组)。I/R组和DEX组切除右肾建立左肾缺血再灌注损伤模型。DEX组在肾缺血前30 min腹腔注射10μg/kg右美托咪定。再灌注24 h后,测定肾脏大小和肾血流阻力(RI),超声造影观察肾皮质灌注,时间-强度曲线定量分析达峰时间(TTP)、峰值强度(PSI)、曲线上升斜率(Grad)和曲线下面积(AUC)。取肾脏观察病理改变。结果与对照组比较,I/R组和DEX组肾大小和病理改变明显,RI增高, TTP延长,PSI和Grad降低,AUC显著增加(P<0.05);与I/R组比较,DEX组肾大小和病理改变明显改善,RI降低,TTP缩短, PSI和Grad增高,AUC减少(P<0.05)。结论超声造影结合时间-强度曲线参数能动态定量分析右美托咪定改善兔肾缺血/再灌注损伤时的微循环灌注。
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关 键 词: | 超声造影 右美托咪定 肾缺血/再灌注损伤 微循环灌注 |
Effects of dexmedetomidine on microcirculatory perfusion in rabbits with renal ischemia/reperfusion injury:quantitative evaluation with contrast-enhanced ultrasound |
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Abstract: | Objective To investigate the effects of dexmedetomidine on renal microcirculatory perfusion in rabbits with renal ischemia/reperfusion (I/R) injury rabbit by quantitative analysis of contrast-enhanced ultrasound (CEUS). Methods Twenty-four New Zealand rabbits were randomly divided into 3 groups (8 in each), including a control group, renal I/R injury group and dexmedetomidine group. In the latter two groups, the right kidney of the rabbits was resected and I/R injury was induced in the left kidney. In dexmedetomidine group, the rabbits received an intraperitoneal dose of 10 μg/kg dexmedetomidine 30 min before renal ischemia, and 24 h after reperfusion, the renal size and renal artery resistance (RI) were measured, and renal cortex perfusion was observed by CEUS. The time-to-peak intensity (TTP), peak signal intensity (PSI), gradient between start frame to peak frame (Grad) and area under the curve (AUC) were quantitatively analyzed using the time-intensity curves. Pathological changes of the kidney were also observed. Results Compared with the control group, the rabbits in I/R and dexmedetomidine groups showed distinct changes of the renal size with obvious renal pathologies. RI, PPT and AUC all increased, and PSI and Grad decreased significantly in I/R and dexmedetomidine groups (P<0.05). Compared with I/R group, obvious improvement of the renal size and renal pathologies were observed in dexmedetomidine group, which showed significantly decreased RI, PPT and AUC and increased PSI and Grad (P<0.05). Conclusion CEUS combined with the time-intensity curve parameters allows quantitative and dynamic analysis of the protective effects of dexmedetomidine on microcirculatory perfusion in rabbits with renal I/R injury. |
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Keywords: | contrast-enhanced ultrasound dexmedetomidine renal ischemia/reperfusion injury microcirculatory perfusion |
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