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广州汉族人群12个Y-STR基因座多态性及法医学应用研究
引用本文:刘超,陈玲,陈晓辉,刘长晖,王慧君.广州汉族人群12个Y-STR基因座多态性及法医学应用研究[J].南方医科大学学报,2007,27(5):599-601.
作者姓名:刘超  陈玲  陈晓辉  刘长晖  王慧君
作者单位:1. 南方医科大学法医学研究所,广东,广州,510515;广州市刑事科学技术研究所,广东,广州,510030
2. 南方医科大学法医学研究所,广东,广州,510515
3. 广州市刑事科学技术研究所,广东,广州,510030
基金项目:国家自然科学基金 , 广东省广州市科技攻关项目
摘    要:目的 研究广州地区汉族人群12个Y-STR基因座的等位基因频率分布、单倍型遗传多样性及其法医学应用价值.方法 采用Powerplex(R)Y System荧光标记复合扩增系统检测401例广州汉族无关个体的12个Y-STR基因座,并用3100遗传分析仪进行基因分型.结果 获得了广州地区汉族无关男性个体的12个Y-STR基因座的频率分布资料,观察到398个单倍型,其基因多样性为0.99997;检测13种动物血样,在特异性区域均无扩增产物;55个父系样本,未观察到突变基因;同一男性个体的不同组织基因分型相同且女性DNA成分不影响Y-STR的扩增分型.结论 该12个Y-STR基因座检测系统具有很高的识别能力,在群体遗传学研究及法医学混合检材的个人识别和父系亲缘关系鉴定中有重要应用价值.

关 键 词:Y染色体  STR  单倍型  遗传多态性  法医物证学
文章编号:1673-4254(2007)05-0599-03
收稿时间:2006-10-19
修稿时间:2006年10月19

Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine
LIU Chao,CHEN Ling,CHEN Xiao-hui,LIU Chang-hui,WANG Hui-jun.Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine[J].Journal of Southern Medical University,2007,27(5):599-601.
Authors:LIU Chao  CHEN Ling  CHEN Xiao-hui  LIU Chang-hui  WANG Hui-jun
Institution:Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. liuchaogz@21cn.com
Abstract:Objective To investigate the allele frequency distribution, gene diversity and haplotype diversity of 12 Y-specific short tandem repeats (STR) in Guangzhou Han population and evaluate their forensic application. Methods Twelve Y-STR loci in 401 unrelated male Han individuals in Guangzhou were amplified with PowerPlex Y System, and the PCR products were detected with 3100 Genetic Analyzer. Results The data of allele distribution and gene diversity of the 12 Y-STR loci were obtained from these individuals. A total of 398 haplotypes were observed and the overall haplotype diversity for the 12 Y-STR loci was 0.99997. The DNA samples of 13 different species of animals were amplified and no specific products were observed. Different tissues from the same individual exhibited the same Y-STR haplotype. Conclusion The 12 Y-STR loci exhibit high genetic polymorphism and are of important value in understanding of the human origin, individual identification for mixed male/female DNA samples and paternity identification.
Keywords:Y chromosome  short tandem repeat loci  haplotype  genetic polymorphism  forensic medicine
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