外源性hCG对子宫内膜癌裸鼠移植瘤生长的影响

目的 观察外源性hCG对子宫内膜癌裸鼠移植瘤生长的影响,探讨hCG在子宫内膜癌治疗中的意义.方法 建立子宫内膜癌裸鼠移植瘤模型,用hCG对裸鼠进行皮下注射,计算成瘤率,称量瘤体质量;并对瘤组织进行HE染色,观察肿瘤瘤组织形态及测量坏死面积;Masson染色观察瘤组织纤维化;免疫组织化学染色观察瘤组织Ki-67表达.结果 两组裸鼠成瘤率均为100%(10/10);与对照组相比,hCG处理组裸鼠移植瘤瘤质量有增高趋势;HE染色可见hCG处理组子宫内膜癌细胞呈腺腔样排列,瘤组织内坏死面积较少而范围较小;Masson染色见hCG处理组胶原纤维粗大而致密,而NS组胶原纤维较纤细而稀疏;Ki-67在两组表达无明显差别.结论 外源性hCG在裸鼠体内实验可促进子宫内膜癌细胞的分化.

Effects of exogenous human chorionic gonadotropin on nude mice bearing endometrial carcinoma

Objective To observe the effects of exogenous human chorionic gonadotropin(hCG)on nude mice bearing transplanted endometrial carcinoma.Methods Human endometrial carcinoma xenograft was transplanted in nude mice,and the effects of hCG injection on the tumor growth was evaluated according to tumorigenesis and xenograft weights.The expression of Ki-67 in the tumor was determined by immunohistochemistry,and HE staining was performed for morphological observation and measurement of the necrosis area in the tumor.The effect of hCG on fibrosis in the tumor was evaluated with Masson staining.Results Compared to normal saline-treated tumor-bearing mice,the mice with hCG treatment showed increased tumor weight.HE staining for tumors in HCG-treatment group visualized tumor cell arrangement in glandular structure with smaller necrosis area,and Masson staining identified thick and compact collagen fibers as compared with the thin and loosely arranged fibers in saline-treated group.No significant difference was found in the Ki-67 expression in the tumors between the two groups.Conclusion Exogenous hCG can promote the differentiation of the endometrial carcinoma cells in vivo.