| 链霉菌胞外多糖、灵芝多糖和米糠多糖对HIV gp41六股螺旋束核心结构形成的影响 |
| |
| 引用本文： | 文晓芸,吴少瑜,徐伟,吕琳,刘叔文,饶进军,张嘉杰,王广发,万山河,吴曙光.链霉菌胞外多糖、灵芝多糖和米糠多糖对HIV gp41六股螺旋束核心结构形成的影响[J].南方医科大学学报,2007,27(10):1498-1500. |
| |
| 作者姓名： | 文晓芸 吴少瑜 徐伟 吕琳 刘叔文 饶进军 张嘉杰 王广发 万山河 吴曙光 |
| |
| 作者单位： | 南方医科大学药学院,广东,广州,10515 |
| |
| 基金项目： | 国家自然科学基金
,
广东省自然科学基金
,
广东省广州市科技攻关项目
,
广东省科技厅科技计划 |
| |
| 摘 要： | 目的 对链霉菌胞外多糖、灵芝多糖和米糠多糖体外抑制HIV gp41六股螺旋束核心结构的形成进行比较研究.方法 采用作用于HIV gp41的HIV融合抑制剂的高通量筛选方法(夹心ELISA法)检测3种多糖对HIV gp41六股螺旋束核心结构形成的抑制作用.结果 和结论链霉菌胞外能有效抑制HIV胞膜糖蛋白gp41六股α-螺旋束核心结构的形成,其半效抑制浓度(IC50)为(145.48±7.25)mg/L-1,而灵芝多糖和米糠多糖不具有这种抑制活性.
|
| 关 键 词： | 链霉菌胞外多糖 灵芝多糖 米糠多糖 HIV gp41六股螺旋束 |
| 文章编号： | 1673-4254（2007）10-1498-03 |
| 修稿时间： | 2007-04-06 |
Inhibitory effect of polysaccharides on the six-α-helix bundle formation of HIV gp41 protein |
| |
| WEN Xiao-yun,WU Shao-yu,Xu Wei,Lü Lin,LIU Shu-wen,RAO Jin-jun,ZHANG Jia-jie,WANG Guang-fa,WAN Shan-he,WU Shu-guang.Inhibitory effect of polysaccharides on the six-α-helix bundle formation of HIV gp41 protein[J].Journal of Southern Medical University,2007,27(10):1498-1500. |
| |
| Authors: | WEN Xiao-yun WU Shao-yu Xu Wei Lü Lin LIU Shu-wen RAO Jin-jun ZHANG Jia-jie WANG Guang-fa WAN Shan-he WU Shu-guang |
| |
| Institution: | School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China |
| |
| Abstract: | OBJECTIVE: To compare the in vitro inhibitory effect of expolysaccharides from Streptomyces, polysaccharides of Ganoderma lucidum and rice bran on six-alpha-helix bundle formation of HIV gp41 protein. METHODS: The amount of six-alpha-helix bundle formed in the presence of N36 and C34 was tested by ELISA in response to treatments with different doses of polysaccharides. RESULTS: Expolysaccharides from Streptomyces potentially inhibited six-alpha-helix bundle formation with the effective concentration (IC(50)) of 145.48-/+7.25 mg /L. Polysaccharides of Ganoderma lucidum and rice bran showed no effect on the six-alpha-helix bundle formation. CONCLUSION: Expolysaccharides from Streptomyces can inhibit the six-alpha-helix bundle formation of HIV gp41, whereas polysaccharides of Ganoderma lucidum and rice bran do not exhibit such activity. |
| |
| Keywords: | exopolysaccharides Streptomyces polysaccharides Ganoderma lucidum polysaccharides rice bran HIV gp41 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
