人脐血单个核细胞诱导分化为神经元样细胞

目的 探讨人脐血单个核细胞体外向神经元样细胞定向诱导分化的条件。方法 无菌条件下采集正常人脐血,分离单个核细胞。用DMEM/F12培养基进行纯化和扩增培养。取扩增5代的间充质干细胞(MSC),分别用β-巯基乙醇(β-ME)、二甲基亚砜(DMSO)、神经条件培养液诱导脐血MSC向神经元样细胞分化。免疫组化法检测脐血MSC的Nestin表达和神经元样细胞特异性标志。结果 脐血原代和2、5代MSC的Nestin表达阳性率分别为6.7%、12.4%和20.8%。神经条件培养液诱导神经元样细胞阳性率最高达36%,β-ME、DMSO分别为33%和25%。结论 脐血MSC具有神经干/祖细胞的特性,在一定条件下能向神经元样细胞分化。

Induced differentiation of human cord blood monocytes into neuron-like cells in vitro

Objective To probe the conditions for inducing human cord blood monocytes to differentiate into neuron-like cells. Methods The mononuclear cells were isolated from human umbilical cord blood samples and plated in 25-mm culture flasks containing DMEM/F12 medium. The fifth passage of mesenchymal stem cells(MSCs) were induced by β-mercaptoe-thanol(β-ME), dimethyl sulfoxide(DMSO) and conditioned medium for neuron induction, respectively, to differentiate into neuron-like cells. The expression of nestin, neuron-specific enolase(NSE) and neurofilament(NF) on the treated cells were detected by immunocytochemical method. Results Nestin expression were found in 6.7% of the primary, 12.4% of the second passage, 20.8% of the fifth passage of MSCs, respectively. Thirty-six percent of cell cultures treated with the conditioned medium for neuron induction were immunoreactive for NSE and NF, and 33% of the cells induced by β-ME and 25% of the cells by DMSO also expressed NSE and NF. Conclusion Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.