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| 载银珊瑚羟基磷灰石的载银量及其细胞相容性的实验研究 | |
| 作者姓名： | Zhang Y Yin QS Zhang Y Li J Chen XQ Fu D |
| 作者单位： | 1. 广州军区广州总医院骨科,广东广州510010;南方医科大学研究生学院,广东广州510515 2. 广州军区广州总医院骨科,广东广州,510010 3. 武警8691部队医院,浙江湖州,313003 |
| 基金项目： | 军队“十一五”科技计划项目(06G045); 广东省科技攻关项目(2003C104004,2004B33101005) |
| 摘 要： | 目的测定载银珊瑚羟基磷灰石(Ag+-CHA)人工骨材料的载银量并观察其与成骨细胞的相容性。方法将自制的珊瑚羟基磷灰石浸泡于相应浓度硝酸银溶液中制得不同浓度的载银人工骨材料,用等离子体质谱分析方法检测各载银材料的载银量;并将小鼠胚胎成骨细胞株(MC3T3-E1)种植于人工骨材料上,用MTT法、倒置相差显微镜、激光共聚焦等观察细胞在材料中的生长情况及其生物相容性。结果 10-2、10-3、5×10-4、10-4、8×10-5、5×10-5 mol/L浓度载银珊瑚羟基磷灰石材料的载银量分别为(4127.67±47.35)、(167.90±11.00)、(83.42±4.51)、(30.20±2.32)、(22.39±4.09)、(15.11±0.55)μg/g;MTT结果表明8×10-5、10-5 mol/LAg+-CHA及其他非载银材料对细胞无明显毒性,可诱导细胞迅速增长。镜下观察发现上述材料上的细胞形态正常,贴壁生长良好。结论低浓度载银珊瑚羟基磷灰石在诱导MC3T3-E1细胞的增殖方面性能优越,有良好的生物相容性,可作为抗感染骨替代材料。 |
| 关 键 词： | 珊瑚羟基磷灰石 生物相容性 MTT比色法 抗感染骨替代材料 银 |
Determination of silver content in silver-loaded coral hydroxyapatite in relation to the biocompatibility |
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| Zhang Y,Yin QS,Zhang Y,Li J,Chen XQ,Fu D.Determination of silver content in silver-loaded coral hydroxyapatite in relation to the biocompatibility[J].Journal of Southern Medical University,2011,31(8):1411-1415. | |
| Authors: | Zhang Yu Yin Qing-shui Zhang Yu Li Jian Chen Xu-qiong Fu Dong |
| Institution: | ZHANG Yu1,2,YIN Qing-shui1,LI Jian1,CHEN Xu-qiong1,FU Dong3 1Department of Orthopedics,Guangzhou General Hospital of Guangzhou Command,Guangzhou 510010,China,2Graduate School of Southern Medical University,Guangzhou 510515,3Department of Orthopedics,8691 Hospital of Armed Police Force,Huzhou 313003 |
| Abstract: | Objective To determine the amount of silver in silver-loaded coral hydroxyapatite(Ag+-CHA) bone substitute and its impact on the biocompatibility of this material with mouse embryonic osteoblast cells.Methods Ag+-CHA was prepared by immersing coral hydroxyapatite in a serial concentration of silver nitrate solutions.The amount of silver in the prepared Ag+-CHA was measured by inductively coupled plasma atomic emission spectrometry(ICP-AES).The viability of MC3T3-E1 cells incubated with Ag+-CHA was measured ... |
| Keywords: | coral hydroxyapatite biocompatibility anti-infection bone substitute silver |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |