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复智散HPLC-UV/ELSD指纹图谱的研究
引用本文:赵敬堃,王德生,段淑荣,王建秀,白晶,李文兰.复智散HPLC-UV/ELSD指纹图谱的研究[J].南方医科大学学报,2007,27(12):1830-1833.
作者姓名:赵敬堃  王德生  段淑荣  王建秀  白晶  李文兰
作者单位:1. 哈尔滨医科大学附属第一医院神经内科,黑龙江,哈尔滨,150001
2. 哈尔滨商业大学药物研究所,黑龙江,哈尔滨,150086
基金项目:国家自然科学基金 , 黑龙江省教育厅科学技术研究项目
摘    要:目的 建立中药复智散提取物HPLC-UV/ELSD指纹图谱.方法 采用HPLC-UV/ELSD串联检测技术,应用AlltechC18(5 μm,4.6mm×250 mm)色谱柱;流动相为乙腈(A)-0.1%乙酸水溶液(B),线性梯度时间洗脱程序(A)为:0,12%;25,20%;30,20%;75,30%;105,40%;120,80%;130,12%;流速:1.0ml/min;柱温:30℃;检测波长:335 nm;飘移管温度80℃;氮气压力25 psi.运用指纹图谱相似度评价软件,对12批复智散提取物指纹图谱进行相似度计算.结果 建立中药复智散提取物指纹图谱并鉴定其5种主要成分;对UV与ELSD图谱互补性进行分析;12批复智散提取物指纹图谱相似度良好.结论 该指纹图谱能同时反映复智散复方中主要化学组分特征,一次进样,可全面、稳定、有效地进行复方中药复智散的质量监督与评价.

关 键 词:高效液相色谱  紫外/蒸发光散射检测  复智散  指纹图谱
文章编号:1673-4254(2007)12-1830-04
修稿时间:2007年10月8日

Fingerprinting of Fuzhisan by high-performance liquid chromatography with ultraviolet and evaporative light scattering detector
ZHAO Jing-kun,WANG De-sheng,DUAN Shu-rong,WANG Jian-xiu,BAI Jing,LI Wen-lan.Fingerprinting of Fuzhisan by high-performance liquid chromatography with ultraviolet and evaporative light scattering detector[J].Journal of Southern Medical University,2007,27(12):1830-1833.
Authors:ZHAO Jing-kun  WANG De-sheng  DUAN Shu-rong  WANG Jian-xiu  BAI Jing  LI Wen-lan
Institution:Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. zjk7486@yahoo.com.cn
Abstract:OBJECTIVE: To establish a method for fingerprinting of Fuzhisan (FZS, a traditional Chinese medicinal preparation) using high-performance liquid chromatography with ultraviolet and evaporative light scattering detector (HPLC-UV/ELSD) to allow simultaneous determination of 5 major constituents in the preparation. METHODS: HPLC-UV/ELSD analysis was performed on water AlltechC18 column (5 microm, 4.6 mm x 250 mm) with a mixture of acetonitrile (A) and 0.1% acetice acid water (B) as the mobile phase. The solvent A gradient for elution was 0, 12%; 25, 20%; 30, 20%; 75, 30%; 105, 40%; 120, 80%; 130, 12%, with the flow rate of 1.0 ml/min; and the column temperature at 30 degrees . The detective wavelength was 335 nm, drift tube temperature was 80 degrees , pressure of nebulizer gas was 25 psi. The similarities between the HPLC-UV/ELSD fingerprints of the 12 extracts were calculated using similarity evaluation software. RESULTS: The fingerprint of FZS was established and the 5 major constituents were identified. The complementarity between the fingerprints of UV and ELSD was analyzed, showing good correlation between 12 batches of FZS. CONCLUSION: The method for fingerprinting can simultaneously characterize the main chemical constituents in FZS and allows stable, effective and comprehensive quality control and evaluation of FZS for a single sample.
Keywords:high-performance liquid chromatography  ultraviolet  evaporative light scattering detection  Fuzhisan  fingerprinting
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