| Ⅰ型胶原支架材料与人脂肪干细胞体外生物相容性研究 |
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| 引用本文： | 张云松,高建华,鲁峰,朱茗,廖云君.Ⅰ型胶原支架材料与人脂肪干细胞体外生物相容性研究[J].南方医科大学学报,2007,27(2):223-225. |
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| 作者姓名： | 张云松 高建华 鲁峰 朱茗 廖云君 |
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| 作者单位： | 1. 暨南大学第四附属医院/广州市红十字会医院整形外科
2. 南方医科大学南方医院整形外科,广东,广州,510515 |
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| 摘 要： | 目的 观察人脂肪干细胞与I型胶原支架材料的生物相容性,为脂肪组织工程选择适宜的种子细胞载体.方法 将人脂肪干细胞与Ⅰ型胶原支架体外培养复合,采用倒置相差显微镜和扫描电镜,观察细胞在支架上黏附、生长及增殖情况并计算细胞黏附率,XTT比色法检测细胞增殖率.结果 脂肪干细胞能良好的在支架上黏附、生长和增殖.结论 Ⅰ型胶原支架材料具有良好的细胞相容性,可作为脂肪组织工程的种子细胞载体.
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| 关 键 词： | 人脂肪干细胞 组织工程 支架 细胞相容性 |
| 文章编号： | 1673-4254（2007）02-0223-03 |
| 收稿时间： | 2006-06-25 |
| 修稿时间： | 2006年6月25日 |
Cellular compatibility of type collagen Ⅰ scaffold and human adipose-derived stem cells |
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| ZHANG Yun-song,GAO Jian-hua,LU Feng,ZHU Ming,LIAO Yun-jun.Cellular compatibility of type collagen Ⅰ scaffold and human adipose-derived stem cells[J].Journal of Southern Medical University,2007,27(2):223-225. |
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| Authors: | ZHANG Yun-song GAO Jian-hua LU Feng ZHU Ming LIAO Yun-jun |
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| Institution: | Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yszhang5201314@163.com |
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| Abstract: | OBJECTIVE: To study the cellular compatibility of type I collagen scaffold and human adipose-derived stem cells (ADSC(S)) in order to explore appropriate scaffold materials for adipose tissue engineering. METHODS: The morphology and function of the ADSC(S) were observed by inverted phase contrast microscope, scanning electron microscope and XTT assay when cocultured type I collagen scaffold with ADSC(S) in vitro. Cells adhesive rates were also calculated. RESULT: ADSC(S) were able to attach, grow and proliferate well on the scaffolds. CONCLUSION: collagen I scaffold exhibits excellent cellular compatibility and can be used as a vehicle for adipose tissue engineering. |
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