| 耐药乳腺癌细胞株MCF-7/ADR中microRNA的分析 |
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| 引用本文： | 崔秀英,郭运杰,姚和瑞.耐药乳腺癌细胞株MCF-7/ADR中microRNA的分析[J].南方医科大学学报,2008,28(10):1813-1815. |
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| 作者姓名： | 崔秀英 郭运杰 姚和瑞 |
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| 作者单位： | 1. 中山大学附属第二医院乳腺外科,广东,广州,510120
2. 中山大学附属第二医院肿瘤科,广东,广州,510120 |
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| 基金项目： | 广东省自然科学基金，广东省医学科学技术研究基金，广东省科技厅科技计划 |
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| 摘 要： | 目的 本研究通检测乳腺癌细胞株MCF-7及其阿霉索耐药株MCF-7/ADR的microRNA的表达差异,探讨microRNA与乳腺癌化疗耐药的关系.方法 MTT法检测MCF-7/ADR相对于其亲本细胞MCF-7的耐药性和耐药倍数;microRNA芯片和RT-PCR的方法比较阿霉素耐药细胞株MCF-7/ADR与其对应的MCF-7乳腺癌细胞株microRNA表达差异.结果 乳腺癌的阿霉素耐药株MCF-7/ADR相对于其亲本细胞系MCF-7对阿霉素的耐药倍数为33.2倍.microRNA芯片的结果显示耐药株MCF-7/ADR与亲本细胞系MCF-7比较有16个microRNA高表达,20个microRNA低表达:RT-PCR的结果进一步证实mir-221、mir222、mir-130a、mir-155在MCF-7/ADR中显著上调.而mir200a、mir-200b、mir-200c、mir-421在MCF-7/ADR中显著下调.结论 MCF-7/ADR与MCF-7的microRNA的表达存在着差异,提示mieroRNA参与乳腺癌化疗耐药,为进一步研究microRNA在乳腺癌耐药机制中的作用打下基础.
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| 关 键 词： | 乳腺癌 多药耐药 |
Analysis of microRNA in drug-resistant breast cancer cell line MCF-7/ADR |
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| CUI Xiu-ying,GUO Yun-jie,YAO He-rui.Analysis of microRNA in drug-resistant breast cancer cell line MCF-7/ADR[J].Journal of Southern Medical University,2008,28(10):1813-1815. |
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| Authors: | CUI Xiu-ying GUO Yun-jie YAO He-rui |
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| Institution: | CUI Xiu-ying1,GUO Yun-jie2,YAO He-rui2 1Department of Breast Surgery,2Department of Oncology,Second Affiliated Hospital of Sun Yat-sen University,Guangzhou 510120,China |
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| Abstract: | Objective To analyze the difference in microRNAs expression between MCF-7 and MCF-7/ADR cells and explore the association between microRNA and drug resistance of breast cancer.Methods The drug resistance of MCF-7/ADR cells was evaluated using MTT assay and flow cytometry.Microarray technique and RT-PCR were used to analyze the differential expressions of the microRNA between MCF-7 and MCF-7/ADR cells.Results The drug resistance index of MCF-7/ADR cells relative to the parental MCF-7 cells was 33.2.The perce... |
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| Keywords: | microRNA |
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