首页 | 本学科首页   官方微博 | 高级检索  
检索        

系统性红斑狼疮患者外周血T细胞TCRβ链CDR3谱系漂移和序列鉴定
引用本文:罗微,马骊,姚新生,邹红云,温茜,阮光萍,王小宁.系统性红斑狼疮患者外周血T细胞TCRβ链CDR3谱系漂移和序列鉴定[J].南方医科大学学报,2006,26(8):1128-1131.
作者姓名:罗微  马骊  姚新生  邹红云  温茜  阮光萍  王小宁
作者单位:1. 南方医科大学生物技术学院分子免疫学研究所,广东,广州,510515
2. 华南理工大学生物科学与工程学院,广东,广州,510641
基金项目:国家重点基础研究发展计划(973计划)
摘    要:目的 探讨系统性红斑狼疮(SLE)患者外周血T细胞TCRβ链CDR3谱系漂移,为SLE的免疫应答机制和个性化治疗研究提供基础。方法 采用免疫扫描谱型分析技术,分析5例正常献血员的CDR3分布特征及5例SLE患者PBMC中T细胞TCRβ链CDR3的优势利用情况,对克隆性增生T细胞的CDR3区进行序列分析。结果 5例正常献血员PBMC TCR BV CDR3谱型均呈高斯分布,5例活动型SLE患者24TCR BV CDR3家族均出现不同的优势表达。对单/寡克隆性增生T细胞B链CDR3区基因进行测序,证实存在不同的CDR3序列。结论 SLE活动期外周血T细胞TCRβ链CDR3谱系出现明显漂移,提示CDR3的选择性表达可能与SLE的免疫发病机理有关。特异应答的T细胞TCRCDR3序列的确定,将为SLE的发病机制研究和个性化治疗提供新的方法与手段。

关 键 词:系统性红斑狼疮  T淋巴细胞受体  互补决定区3  免疫扫描谱型分析  基因扫描
文章编号:1673-4254(2006)08-1128-04

Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with systemic lupus erythematosus
LUO Wei,MA Li,YAO Xin-sheng,ZOU Hong-yun,WEN Qian,RUAN Guang-ping,WANG Xiao-ning.Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with systemic lupus erythematosus[J].Journal of Southern Medical University,2006,26(8):1128-1131.
Authors:LUO Wei  MA Li  YAO Xin-sheng  ZOU Hong-yun  WEN Qian  RUAN Guang-ping  WANG Xiao-ning
Institution:Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China. LuoWei 421@163.com
Abstract:OBJECTIVE: To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus. METHODS: Immunoscope spectratyping techniques was used to analyze the distribution of TCRbeta chain CDR3 in 5 normal blood donors and the dominant CDR3 in the PBMCs in 5 SLE patients. Sequence analysis of the CDR3 region in monoclonal or oligoclonal T cells was performed. RESULTS: The spectratypes of TCR BV gene CDR3 region showed Gaussian distribution in the 5 normal blood donors. The 5 SLE patients, however, displayed anomalous proliferation and oligoclonal expansion of the T cells was observed in different TCR BV families with different CDR3 sequences. CONCLUSION: Noticeable drift of TCRbeta chain CDR3 can be seen in active SLE, indicating possible association of selective expression of TCR with immune pathogenesis in SLE. Determination of specific TCR CDR3 sequence provides a new means for studying the pathogenesis and personalized treatment of SLE.
Keywords:systemic lupus erythematosus  T cell receptor  complementaritydetermining region 3  immunoscope spectratyping  gene scanning
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号