小干扰RNA沉默OCT4 基因表达对胰腺癌细胞株PANC1增殖与凋亡的影响

摘要：目的运用RNA干扰(RNA inteference，RNAi)技术阻断胰腺癌细胞株PANC1中OCT4基因表达，并研究该基因沉默
后对细胞增殖与凋亡的影响。方法利用阳离子脂质体LipofectamineTM2000 将化学合成的人Octamer binding factor 4
（OCT4）的小干扰RNA转染入胰腺癌细胞株PANC1 中。RT-PCR法测定胰腺癌细胞内OCT4 mRNA的表达。Western
Blot测定OCT4、PARP。CCK8法测定细胞生长曲线观察细胞增殖的抑制情况。流式细胞仪测定细胞凋亡率的变化。结
果化学合成的人OCT4siRNA能有效地抑制PANC1细胞中OCT4的表达(P<0.05)。OCT4 siRNA组中细胞增殖减慢，凋
亡率较对照组明显增加(P<0.05)。干扰OCT4能够激活PARP的表达。结论体外实验初步证明OCT4基因在胰腺癌细
胞株增殖分化与凋亡方面扮演重要角色。通过沉默其表达可抑制胰腺癌细胞株PANC1增殖并诱导其凋亡。

Small interfering RNA-mediated OCT4 gene silencing inhibits the proliferation and induces apoptosis of pancreatic cancer cell line PANC1

Objective To observe the effects of small interfering RNA(siRNA) -mediated silencing of octamer binding factor 4(OCT4) gene on the proliferation and apoptosis of pancreatic carcinoma cell line PANC1 in vitro. Methods Chemically synthesized siRNA against human OCT4 was transfected into PANC1 cells via LipofectamineTM2000. The expression of OCT4 mRNA in the cells was detected using RT-PCR,and the protein expressions of OCT4 and PARP were assayed using Western blotting. The changes in the cell proliferation we...