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| 实时荧光定量RT-PCR分析非小细胞肺癌SATB1 的表达和临床病理意义 | |
| 引用本文： | 周来勇,刘芳,童健,陈群请,张福伟,郭琳琅.实时荧光定量RT-PCR分析非小细胞肺癌SATB1 的表达和临床病理意义[J].南方医科大学学报,2009,29(3):534. |
| 作者姓名： | 周来勇 刘芳 童健 陈群请 张福伟 郭琳琅 |
| 作者单位： | 1. 南方医科大学珠江医院胸心外科,广东,广州,510282 2. 南方医科大学肿瘤研究所,广东,广州,510282 3. 南方医科大学珠江医院病理科,广东,广州,510282 |
| 摘 要： | 目的 检测非小细胞肺癌组织中SATB1 mRNA的表达,探讨SATB1表达与非小细胞肺癌发生、发展的关系及其临床病理意义. 方法 用TRIZOL提取非小细胞肺癌组织和正常肺组织总RNA后,将其反转录为cDNA,以实时荧光定量RT-PCR方法 检测非小细胞肺癌组织及正常肺组织中SATB1 mRNA的表达,分析SATB1基因表达与临床病理参数的相关性.结果 癌组织中SATB1 mRNA表达量为正常组织13倍,两者差异显著(P<0.001),其中有、无转移组分别为正常组织23.63倍和5.57倍.结论 SATB1 mRNA的表达水平可能与非小细胞肺癌发生发展及淋巴转移有关,有望成为判断非小细胞肺癌预后的一个指标. |
| 关 键 词： | 非小细胞肺癌 实时荧光定量RT-PCR |
Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer |
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| ZHOU Lai-yong,LIU Fang,TONG Jian,CHEN Qun-qing,ZHANG Fu-wei.Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer[J].Journal of Southern Medical University,2009,29(3):534. | |
| Authors: | ZHOU Lai-yong LIU Fang TONG Jian CHEN Qun-qing ZHANG Fu-wei |
| Institution: | ZHOU Lai-yong1,LIU Fang2,TONG Jian1,CHEN Qun-qing1,ZHANG Fu-wei1 1Department of Cardiothoracic Surgery,Zhujiang Hospital,3Department of Pathology,2Institute of Cancer Research,Southern Medical University,Guangzhou 510282,China |
| Abstract: | Objective To detect the expression of AT-rich sequence-binding protein(SATB1) mRNA in non-small cell lung cancer(NSCLC) and explore the role of SATB1 in the development of NSCLC.Methods The total RNA was extracted from NSCLC tissues and normal lung tissues and reverse transcribed into cDNA.Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of SATB1 mRNA these tissues.Results The expression of SATB1 mRNA was 13-fold higher in NSCLC tissues than in normal lung tissues(P<0.00... |
| Keywords: | SATB1 mRNA |
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