| 高血压大鼠阴茎海绵体平滑肌细胞表型转化 |
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| 引用本文： | 韦安阳,吴威武,吴浩彬,叶挺宇,万波.高血压大鼠阴茎海绵体平滑肌细胞表型转化[J].南方医科大学学报,2009,29(5). |
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| 作者姓名： | 韦安阳 吴威武 吴浩彬 叶挺宇 万波 |
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| 作者单位： | 南方医科大学南方医院惠侨楼一病区,广东,广州,510515 |
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| 基金项目： | 全军十五科技计划基金，广东省科技计划项目 |
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| 摘 要： | 目的 探讨高血压大鼠阴茎海绵体平滑肌是否存在表型转化及对勃起功能的影响.方法 16周龄雄性自发性高血压大鼠(SHR)及其同系正常血压大鼠(WKY),测量尾动脉收缩压,颈部皮下注射阿朴吗啡后检测阴茎勃起功能,然后将大鼠分为高血压勃起功能障碍组(HBP&ED)、高血压组(HBP)和正常对照组(Control).免疫组化染色和彩色图文分析系统分析各组大鼠阴茎海绵体中平滑肌细胞碱性调宁蛋白(calponin 1)和骨桥蛋白(osteopontin,OPN)的表达,实时荧光定量RT-PCR分析各组大鼠阴茎海绵体平滑肌细胞calponin 1 mRNA和OPN mRNA的相对表达量.结果 在大鼠阴茎海绵体中calponin 1和calponin 1 mRNA表达情况:HBP&ED组低于HBP组(P=0.021,P=0.006)和control组(P=0.000,P=0.001),HBP组低于control组(P=0.000,P=0.015);OPN和OPN mRNA表达情况:HBP&ED组高于HBP组(P=0.000,P=0.000)及control组(P=0.000,P=0.000),HBP组高于control组(PP=0.013,p=0.000).结论 高血压大鼠阴茎海绵体平滑肌细胞存在表型由收缩型向合成型转化,这种转化达到一定程度最终可能导致大鼠勃起功能障碍.
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| 关 键 词： | 自发性高血压 勃起功能障碍 阴茎海绵体 平滑肌细胞 表型转化 碱性调宁蛋白 骨桥蛋白 |
Phenotypic transformation of corpus cavernosum smooth muscle cells in hypertensive rats |
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| WEI An-yang,WU Wei-wu,WU Hao-bin,YE Ting-yu,WAN Bo.Phenotypic transformation of corpus cavernosum smooth muscle cells in hypertensive rats[J].Journal of Southern Medical University,2009,29(5). |
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| Authors: | WEI An-yang WU Wei-wu WU Hao-bin YE Ting-yu WAN Bo |
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| Institution: | WEI An-yang,WU Wei-wu,WU Hao-bin,YE Ting-yu,WAN Bo First Division of Huiqiao Department,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To validate the hypothesis that the phenotypic transformation occurs in the smooth muscle cells in the corpus cavernosum of hypertensive rat and explore its impact on the erectile function of rats. Method Eighteen 16-week-old male spontaneously hypertensive rats (SHR) and 10 syngeneic normotensive rats (WKY) were used in this experiment. After measurement of systolic blood pressure of the caudal artery and examination of the erectile function with subcutaneous injection of apomorphine (APO),the ra... |
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| Keywords: | spontaneous hypertension erectile dysfunction corpus cavernosum smooth muscle cells phenotype modulation calponin 1 osteopontin |
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