具有肝转移倾向的结肠癌细胞亚系的筛选与鉴定

目的 筛选人结肠癌SW480细胞中具有特定肝脏转移倾向的亚克隆,比较它与母系生物学特性的差异,为结肠癌肝转移机制的研究提供基本的实验材料.方法 采用外科原位接种、组织块细胞培养法从裸鼠体内获得SW480细胞系中具有肝脏转移倾向的亚克隆SW480/M5;体内体外实验验证它与母系细胞在生物学特性上的差异.结果 体内实验证明SW480具有广泛转移的潜能,而SW480/M5细胞只具有向肝脏转移的潜能;SW480/M5细胞原位瘤的质量较SW480细胞大且皮下瘤生长速度较快:体外实验证明SW480/M5细胞的克隆形成能力,体外增殖能力、体外运动及侵袭能力较SW480细胞强,而对FN的粘附能力较SW480细胞弱.结论 从结肠癌细胞系SW480中筛选出的细胞亚系SW480/M5转移方向明确,是结肠癌肝转移机制研究的理想细胞模型.

Isolation and characterization of human colorectal cancer cell subline with unique metastatic potential in the liver

Objective To isolate a subclone from human colorectal cancer cell line SW480 with unique metastatic potential in the liver. Methods Human colorectal cancer cells SW480 were implanted into BALB/c nude mice, and the hepatic metastatic lesions were harvested, and the tumor tissue blocks were re-implantated into nude mice for a second round of in vivo selection. The same procedure was repeated 5 times until a new cell subline, designated as SW480/MS, was established, whose biological behaviors was investigated both in vivo and in vitro. Results After orthotopic implantation of the tumor tissue into the colon of nude mouse for 28 days, widespread loco-regional and distant metastases occurred in mice inoculated with SW480 tissue, and those inoculated with SW480/M5 tissue had hepatic metastasis only. Compared with the parental cells, SW480/M5 cells were characterized by greater clonality, reproductive activity, motor ability, invasiveness, and weaker adhesive capacity to fibronectin. Conclusion A human colorectal cancer cell subline with unique liver metastatic potential has been established by in vivo selection to serve as a new model for study ing colorectal cancer metastasis.