高低转移人大肠癌细胞系基因表达谱差异

目的用自制肿瘤转移相关基因cDNA微阵列研究人大肠癌高转移细胞系Lovo、SW620及低转移细胞系SW480、LS174T的基因表达谱差异,筛选与大肠癌转移相关的特异基因。方法分别提取4种大肠癌细胞系及对照的正常大肠上皮组织的mRNA,逆转录成cDNA探针,经cy3和cy5标记后分别与含有399个肿瘤转移相关基因的微阵列杂交,用专用软件分析杂交信号强度。结果高转移细胞系与低转移细胞系的基因表达谱有显著差异,其中有104个基因的表达值有意义,包括上调基因44个、下调基因60个。结论大肠癌转移是由其发生过程中多个基因表达的复杂变化决定的,高转移细胞系与低转移细胞系比较存在差异的基因可能与高转移特性有关。

Differences in gene expression profile between high and low metastatic human colorectal cancer cell lines

Objective To analyze the gene expression profiles in colorectal cancer cell lines including high metastatic LoVo and SW620 cell lines and low metastatic SW480 and LS174T cells lines using cDNA microarray for tumor metastasis-associated gene and to screen specific colorectal cancer metastasis-associated genes. Methods The cDNAs reversely transcribed from the mRNA derived respectively from the 4 cell lines and normal colorectal tissues were labeled with cy3 and cy5 prior to hybridization with the microarray containing 399 genes. The acquired image was analyzed by software. Results There were significant differences in gene expression profiles between high- and low- metastatic cell lines, and 44 up-regulated genes and 60 down-regulated genes were found to have statistical significance. Conclusions The complex alterations in gene expression underlie the development of metastasis of colorectal cancer. The genes that are expressed differentially in high- and low- metastatic cell lines are probably related to the high metastatic potentials.